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Home > Products > 110 161

Syntaxin16 antibody - 110 161 K.D.

Syntaxin16 is a SNARE protein involved in trans-Golgi network trafficking
Mouse monoclonal purified IgG
Cat. No.: 110 161
Amount: 100 µg
Price: $415.00
Cat. No. 110 161 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: not recommended
IHC: not recommended
IHC-P: not tested yet
ELISA: yes (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Clone 148.6
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 302 from rat Syntaxin16 (UniProt Id: A0A0G2K528)
Reactivity Reacts with: rat (A0A0G2K528).
No signal: zebrafish.
Other species not tested yet.
Specificity K.D. validated PubMed: 23626741
Matching control protein/peptide 110-16P
Remarks

ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 110 163 as detector antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet 110_161.pdf

References for Syntaxin16 - 110 161

Knockout of Syntaxin-4 in 3T3-L1 adipocytes reveals new insight into GLUT4 trafficking and adiponectin secretion.
Black HL, Livingstone R, Mastick CC, Al Tobi M, Taylor H, Geiser A, Stirrat L, Kioumourtzoglou D, Petrie JR, Boyle JG, Bryant NJ, et al.
Journal of cell science (2021) : . 110 161 WB; tested species: mouse
Syntaxin 16 regulates lumen formation during epithelial morphogenesis.
Jung JJ, Inamdar SM, Tiwari A, Ye D, Lin F, Choudhury A
PloS one (2013) 84: e61857. 110 161 WB; KD verified; tested species: rabbit
EHD2 regulates plasma membrane integrity and downstream insulin receptor signalling events.
Neuhaus M, Fryklund C, Taylor H, Borreguero-Muñoz A, Kopietz F, Ardalani H, Rogova O, Stirrat L, Bremner SK, Spégel P, Bryant NJ, et al.
Molecular biology of the cell (2023) : mbcE23030078. 110 161 IP; tested species: mouse
Cat. No.: 110 161
Amount: 100 µg
Price: $415.00
Knockout of Syntaxin-4 in 3T3-L1 adipocytes reveals new insight into GLUT4 trafficking and adiponectin secretion.
Black HL, Livingstone R, Mastick CC, Al Tobi M, Taylor H, Geiser A, Stirrat L, Kioumourtzoglou D, Petrie JR, Boyle JG, Bryant NJ, et al.
Journal of cell science (2021) : . 110 161 WB; tested species: mouse
Syntaxin 16 regulates lumen formation during epithelial morphogenesis.
Jung JJ, Inamdar SM, Tiwari A, Ye D, Lin F, Choudhury A
PloS one (2013) 84: e61857. 110 161 WB; KD verified; tested species: rabbit
EHD2 regulates plasma membrane integrity and downstream insulin receptor signalling events.
Neuhaus M, Fryklund C, Taylor H, Borreguero-Muñoz A, Kopietz F, Ardalani H, Rogova O, Stirrat L, Bremner SK, Spégel P, Bryant NJ, et al.
Molecular biology of the cell (2023) : mbcE23030078. 110 161 IP; tested species: mouse
WB
Background

Syntaxin 16, a member of the SNARE family of proteins, localizes to the Golgi stack. It has been shown to be involved in trans-Golgi network trafficking and to interact with VAMP 3, VAMP 4 and VAMP 8.
Four splice variants (syntaxin 16a, b, c, d) have been described, which may have different roles in intracellular trafficking. The splice variant c is the shortest and localizes to the cytoplasm.
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