|Cat. No. 108 111||100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery
ICC: 1 : 100 up to 1 : 1000 (see remarks) gallery
IHC: 1 : 50 up to 1 : 500 gallery
IHC-P/FFPE: not tested yet
|Subtype||IgG2a (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 215 from rat Rab5 (UniProt Id: O88565)|
Reacts with: human (Q9UJ41), rat (M0RC99), mouse (Q9JM13).
Other species not tested yet.
|Specificity||Specific for rab 5 (probably only rab 5a). No cross-reactivity to other rab proteins.|
This antibody was used very successfully for immunoisolation of early endosomes and for the differentiation of early endosomes from related trafficking organelles in neurons and nonneuronal cells.