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Rab3a antibody - 107 108 K.O.

Rab3 proteins are the major Rab isoforms in brain. They are highly enriched on synaptic vesicles
Rabbit monoclonal recombinant IgG
Cat. No.: 107 108
Amount: 50 µg
Price: $415.00
Cat. No. 107 108 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 5000 gallery  
IHC: 1 : 500 up to 1 : 2000 gallery  
IHC-P: not recommended
Clone Rb42.2
Subtype IgG1 (κ light chain)
Immunogen Full length rat recombinant Rab3a (UniProt Id: P63012)
Epitop Epitop: AA 95 to 151 from rat Rab3a (UniProt Id: P63012)
Reactivity Reacts with: mouse (P63011), rat (P63012), human (P20336).
Other species not tested yet.
Specificity K.O. validated

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 42.2. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 107_108.pdf
Cat. No.: 107 108
Amount: 50 µg
Price: $415.00

Rab 3 is a member of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. Four related isoforms of Rab 3 are known (Rab 3a, 3b, 3c, and 3d). Rab 3a and 3c are predominantly expressed in neurons and neuroendocrine cells where they are localized to synaptic vesicles. Unlike the integral membrane proteins of synaptic vesicles, Rab 3a/c is absent from the Golgi complex and thus does not result in immunostaining of the axo-dendritic region as sometimes seen with e.g. synaptophysin, synaptobrevin/VAMP, or synaptogyrin.
Rab 3b and 3d are expressed in non-neuronal tissues such as adipocytes and the exocrine pancreas (3d). It has been shown that overexpression of Rab 3 inhibits Ca2+ regulated excocytosis and converts it into an constitutive Ca2+ independent exocytosis mechanism.