|Cat. No. 106 308||50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery
IP: not tested yet
ICC: 1 : 1000 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 2000 gallery
|Subtype||IgG2 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 704 from rat Synapsin1 (UniProt Id: P09951)|
Epitop: AA 435 to 475 from rat Synapsin1 (UniProt Id: P09951)
Reacts with: human (P17600), rat (P09951), mouse (O88935), mammals.
Weaker signal: chicken, zebrafish, other vertebrates.
Other species not tested yet.
|Specificity||Specific for synapsin 1a and 1b independent of phosphorylation state. K.O. K.O.|
This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 46.1. The constant regions of the heavy and light chains have been replaced by Guinea pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
Synapsins are neuron-specific phosphoproteins that are exclusively associated with small synaptic vesicles, with little or no expression in other tissues including neuroendocrine cells. In mammals, three distinct synapsin genes (synapsin 1, 2, and 3) encode more than eight neuronal isoforms.
Synapsin 1 is one of the most specific markers of synapses throughout the central and peripheral nervous system. In addition to synaptic nerve terminals, the protein is also present in certain sensory nerve endings. It is expressed in two splice variants (synapsin 1a and synapsin 1b). Synapsin 1 interacts with vesicle membranes as well as with actin and spectrin.
Synapsin 2 is expressed in the nervous system and also two splice variants were described so far, while synapsin 3 shows a more restricted expression pattern and is mainly found in the hypocampus.
Synapsins are major phosphoproteins and are substrates for several protein kinases such as PKA, CaMK I and CaMK II. Synapsin 1 is widely used as reference substrate for calmodulin-dependent protein kinases.