Cat. No.: 106 211
Amount: 100 µg
Price:
$415.00
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| Cat. No. 106 211 |
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Clone | 27E3 |
| Subtype | IgG1 (κ light chain) |
| Immunogen | Synthetic peptide corresponding to AA 440 to 458 from rat Synapsin2 (UniProt Id: Q63537-1) |
| Reactivity |
Reacts with: human (Q92777), rat (Q63537), mouse (Q64332). No signal: zebrafish. Other species not tested yet. |
| Specificity | K.O. validated |
| Matching control protein/peptide | 106-2P |
| Remarks |
WB: This antibody is less sensitive than the polyclonal rabbit antibody (cat. no. 106 203). Enriched synaptic vesicles are recommended for westernblotting. |
| Data sheet | 106_211.pdf |
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Synapsins are neuron-specific phosphoproteins that are exclusively associated with small synaptic vesicles, with little or no expression in other tissues including neuroendocrine cells. In mammals, three distinct synapsin genes (synapsin 1, 2 and 3) encode more than eight neuronal isoforms.
Synapsin 1 is one of the most specific markers of synapses throughout the central and peripheral nervous system. In addition to synaptic nerve terminals, the protein is also present in certain sensory nerve endings. It is expressed in two splice variants (synapsin 1a and synapsin 1b). Synapsin 1 interacts with vesicle membranes as well as with actin and spectrin.
Synapsin 2 is expressed in the nervous system and also two splice variants were described so far, while synapsin 3 shows a more restricted expression pattern and is mainly found in the hypocampus.
Synapsins are major phosphoproteins and are substrates for several protein kinases such as PKA, CaMK I and CaMK II. Synapsin 1 is widely used as reference substrate for calmodulin-dependent protein kinases.