Synaptic Systems - Synapsin1

Cat. No.: 106 104

Amount: 100 µl

Price: $375.00

Cat. No. 106 104	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 500 gallery EM: yes
Immunogen	Synthetic peptide corresponding to AA 445 to 462 from mouse Synapsin1 (UniProt Id: O88935)
Reactivity	Reacts with: mouse (O88935), rat (P09951). Other species not tested yet.
Specificity	Specific for synapsins 1a and 1b, no cross-reactivity to synapsin 2a/b. K.O. validated
Matching control protein/peptide	106-1P
Data sheet	106_104.pdf

References for Synapsin1 - 106 104

WB (3)
ICC (6)
IHC (6)
EM (1)

Analysis of neurexin-neuroligin complexes supports an isoform-specific role for beta-neurexin-1 dysfunction in a mouse model of autism.
Arias-Aragón F, Robles-Lanuza E, Sánchez-Gómez Á, Martinez-Mir A, Scholl FG
Molecular brain (2025) 181: 20. 106 104 WB, ICC; tested species: human,rat

Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function.
Parra-Rivas LA, Madhivanan K, Aulston BD, Wang L, Prakashchand DD, Boyer NP, Saia-Cereda VM, Branes-Guerrero K, Pizzo DP, Bagchi P, Sundar VS, et al.
Neuron (2023) 11124: 4006-4023.e10. 106 104 WB; tested species: mouse

Rho-kinase inhibition by fasudil modulates pre-synaptic vesicle dynamics.
Saal KA, Warth Pérez Arias C, Roser AE, Christoph Koch J, Bähr M, Rizzoli SO, Lingor P
Journal of neurochemistry (2020) : . 106 104 WB; tested species: rat

Rasopathy-Associated Mutation Ptpn11D61Y has Age-Dependent Effect on Synaptic Vesicle Recycling.
Guhathakurta D, Selzam F, Petrušková A, Weiss EM, Akdaş EY, Montenegro-Venegas C, Zenker M, Fejtová A
Cellular and molecular neurobiology (2024) 441: 77. 106 104 ICC; tested species: mouse

Bassoon controls synaptic vesicle release via regulation of presynaptic phosphorylation and cAMP.
Montenegro-Venegas C, Guhathakurta D, Pina-Fernandez E, Andres-Alonso M, Plattner F, Gundelfinger ED, Fejtova A
EMBO reports (2022) 238: e53659. 106 104 ICC; tested species: mouse

Action potential-coupled Rho GTPase signaling drives presynaptic plasticity.
O'Neil SD, Rácz B, Brown WE, Gao Y, Soderblom EJ, Yasuda R, Soderling SH
eLife (2021) 10: . 106 104 ICC; tested species: mouse

Semaphorin 3A controls enteric neuron connectivity and is inversely associated with synapsin 1 expression in Hirschsprung disease.
Gonzales J, Le Berre-Scoul C, Dariel A, Bréhéret P, Neunlist M, Boudin H
Scientific reports (2020) 101: 15119. 106 104 ICC; tested species: rat

Plug-and-Play Protein Modification Using Homology-Independent Universal Genome Engineering.
Gao Y, Hisey E, Bradshaw TWA, Erata E, Brown WE, Courtland JL, Uezu A, Xiang Y, Diao Y, Soderling SH
Neuron (2019) : . 106 104 ICC; tested species: mouse

Loss of microglial MCT4 leads to defective synaptic pruning and anxiety-like behavior in mice.
Monsorno K, Ginggen K, Ivanov A, Buckinx A, Lalive AL, Tchenio A, Benson S, Vendrell M, D'Alessandro A, Beule D, Pellerin L, et al.
Nature communications (2023) 141: 5749. 106 104 IHC; tested species: mouse

FMRP phosphorylation modulates neuronal translation through YTHDF1.
Zou Z, Wei J, Chen Y, Kang Y, Shi H, Yang F, Shi Z, Chen S, Zhou Y, Sepich-Poore C, Zhuang X, et al.
Molecular cell (2023) 8323: 4304-4317.e8. 106 104 IHC; tested species: human

An adult-stage transcriptional program for survival of serotonergic connectivity.
Kitt MM, Tabuchi N, Spencer WC, Robinson HL, Zhang XL, Eastman BA, Lobur KJ, Silver J, Mei L, Deneris ES
Cell reports (2022) 393: 110711. 106 104 IHC; tested species: mouse

Expression and impact of Lsamp neural adhesion molecule in the serotonergic neurotransmission system.
Bregin A, Kaare M, Jagomäe T, Karis K, Singh K, Laugus K, Innos J, Leidmaa E, Heinla I, Visnapuu T, Oja EM, et al.
Pharmacology, biochemistry, and behavior (2020) 198: 173017. 106 104 IHC; tested species: mouse

Short-term plasticity at cerebellar granule cell to molecular layer interneuron synapses expands information processing.
Dorgans K, Demais V, Bailly Y, Poulain B, Isope P, Doussau F
eLife (2019) 8: . 106 104 IHC, EM; tested species: mouse

Localization of group II and III metabotropic glutamate receptors at pre- and postsynaptic sites of inner hair cell ribbon synapses.
Klotz L, Wendler O, Frischknecht R, Shigemoto R, Schulze H, Enz R
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2019) : fj201901543R. 106 104 IHC; tested species: mouse

Cat. No.: 106 104

Amount: 100 µl

Price: $375.00

References
WB (3)
ICC (6)
IHC (6)
EM (1)

ICC

IHC

IHC-P

Background

Synapsins are neuron-specific phosphoproteins that are exclusively associated with small synaptic vesicles, with little or no expression in other tissues including neuroendocrine cells. In mammals, three distinct synapsin genes (synapsin1, 2, and 3) encode more than eight neuronal isoforms.
Synapsin1 is one of the most specific markers of synapses throughout the central and peripheral nervous system. In addition to synaptic nerve terminals, the protein is also present in certain sensory nerve endings. It is expressed in two splice variants (synapsin 1a and synapsin 1b). Synapsin1 interacts with vesicle membranes as well as with actin and spectrin.
Synapsin2 is expressed in the nervous system and also two splice variants were described so far, while synapsin3 shows a more restricted expression pattern and is mainly found in the hypocampus.
Synapsins are major phosphoproteins and are substrates for several protein kinases such as PKA, CaMK I and CaMK II. Synapsin1 is widely used as reference substrate for calmodulin-dependent protein kinases.

1 ) Hosaka M et al., Neuron (1999)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Rosahl TW et al., Nature (1995)
4 ) Südhof TC et al., Nature (1995)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

OTHER

Preabsorbtion

Other products for this target

Synapsin1 antibody - 106 104 K.O.

References for Synapsin1 - 106 104