Cat. No.: 106 011C2
Amount: 100 µg
Price:
$475.00
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| Cat. No. 106 011C2 |
100 µg purified IgG, lyophilized, fluorescence-labeled with
Cyanine 2.
Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications | |
| Label | Cyanine 2 |
| Clone | 46.1 |
| Subtype | IgG1 |
| Immunogen | full-length recombinant rat Synapsin1 (UniProt Id: P09951) |
| Epitop |
AA 435 to 475 from rat Synapsin1 (UniProt Id: P09951) |
| Reactivity |
Reacts with: human (P17600), rat (P09951), mouse (O88935), mammals. Weaker signal: zebrafish, chicken, other vertebrates. Other species not tested yet. |
| Specificity | Specific for synapsin 1a and 1b independent of phosphorylation state. K.O. validated |
| Data sheet | 106_011c2.pdf |
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Synapsins are neuron-specific phosphoproteins that are exclusively associated with small synaptic vesicles, with little or no expression in other tissues including neuroendocrine cells. In mammals, three distinct synapsin genes (synapsin1, 2, and 3) encode more than eight neuronal isoforms.
Synapsin1 is one of the most specific markers of synapses throughout the central and peripheral nervous system. In addition to synaptic nerve terminals, the protein is also present in certain sensory nerve endings. It is expressed in two splice variants (synapsin 1a and synapsin 1b). Synapsin1 interacts with vesicle membranes as well as with actin and spectrin.
Synapsin2 is expressed in the nervous system and also two splice variants were described so far, while synapsin3 shows a more restricted expression pattern and is mainly found in the hypocampus.
Synapsins are major phosphoproteins and are substrates for several protein kinases such as PKA, CaMK I and CaMK II. Synapsin1 is widely used as reference substrate for calmodulin-dependent protein kinases.