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Synapsin 1 - 106 008

Peripheral synaptic vesicle protein; substrate for several protein kinases
Monoclonal Recombinant rabbit purified IgG
Cat. No.: 106 008
Amount: 50 µg
Price: 415.00 €
Cat. No. 106 008 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: yes
ICC: 1 : 1000 gallery  
IHC: 1 : 200 gallery  
IHC-P/FFPE: 1 : 1000 gallery  
Clone Rb46.1
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 704 from rat Synapsin1 (UniProt Id: P09951)
Epitop Epitop: AA 435 to 475 from rat Synapsin1 (UniProt Id: P09951)
Reactivity Reacts with: human (P17600), rat (P09951), mouse (O88935), mammals.
Weaker signal: chicken, zebrafish, other vertebrates.
Other species not tested yet.
Specificity Specific for synapsin 1a and 1b independent of phosphorylation state. K.O.
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 46.1. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 106_008.pdf
Cat. No.: 106 008
Quantity: 50 µg
Price: 415.00 €
Background

Synapsins are neuron-specific phosphoproteins that are exclusively associated with small synaptic vesicles, with little or no expression in other tissues including neuroendocrine cells. In mammals, three distinct synapsin genes (synapsin 1, 2, and 3) encode more than eight neuronal isoforms.
Synapsin 1 is one of the most specific markers of synapses throughout the central and peripheral nervous system. In addition to synaptic nerve terminals, the protein is also present in certain sensory nerve endings. It is expressed in two splice variants (synapsin 1a and synapsin 1b). Synapsin 1 interacts with vesicle membranes as well as with actin and spectrin.
Synapsin 2 is expressed in the nervous system and also two splice variants were described so far, while synapsin 3 shows a more restricted expression pattern and is mainly found in the hypocampus.
Synapsins are major phosphoproteins and are substrates for several protein kinases such as PKA, CaMK I and CaMK II. Synapsin 1 is widely used as reference substrate for calmodulin-dependent protein kinases.