Synaptic Systems - Synapsin1

Cat. No.: 106 008

Amount: 50 µg

Price: $425.00

Methods for shipping live primary cortical and hippocampal neuron cultures from postnatal mice.
Sammoura FM, Popova D, Morris A, Hart RP, Richardson JR
Current research in neurobiology (2023) 4: 100069. 106 008 ICC; tested species: mouse

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 106 008 DNA-PAINT; tested species: rat

WB

ICC

IHC

IHC-P

IHC-G

ExM

Background

Synapsins are neuron-specific phosphoproteins that are exclusively associated with small synaptic vesicles, with little or no expression in other tissues including neuroendocrine cells. In mammals, three distinct synapsin genes (synapsin1, 2, and 3) encode more than eight neuronal isoforms.
Synapsin1 is one of the most specific markers of synapses throughout the central and peripheral nervous system. In addition to synaptic nerve terminals, the protein is also present in certain sensory nerve endings. It is expressed in two splice variants (synapsin 1a and synapsin 1b). Synapsin1 interacts with vesicle membranes as well as with actin and spectrin.
Synapsin2 is expressed in the nervous system and also two splice variants were described so far, while synapsin3 shows a more restricted expression pattern and is mainly found in the hypocampus.
Synapsins are major phosphoproteins and are substrates for several protein kinases such as PKA, CaMK I and CaMK II. Synapsin1 is widely used as reference substrate for calmodulin-dependent protein kinases.

1 ) Hosaka M et al., Neuron (1999)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Rosahl TW et al., Nature (1995)
4 ) Südhof TC et al., Nature (1995)

Protocols

WB

IP

Standard protocol

ICC

IHC

IHC-P

Other products for this target

Cat. No. 106 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery IP: yes ICC: 1 : 1000 gallery IHC: 1 : 200 gallery IHC-P: 1 : 1000 gallery IHC-G: (see remarks) gallery ExM: yes (see remarks) gallery DNA-PAINT: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence. Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section. In antibody-based DNA-PAINT (Point Accumulation in Nanoscale Topography), a short oligomeric docking strand is coupled to a specific antibody. The transient association of the fluorophore to the antibody is mediated by the pairing of a short fluorescently labeled complementary imager DNA strand. DNA-PAINT allows super-resolution imaging and the imaging of a huge number of antibodies on the same biological sample in a single multiplex experiment. For additional experimental details refer to the Remarks section.
Clone	Rb46.1
Subtype	IgG1 (κ light chain)
Immunogen	full-length recombinant rat Synapsin1 (UniProt Id: P09951)
Epitop	AA 435 to 475 from rat Synapsin1 (UniProt Id: P09951)
Reactivity	Reacts with: human (P17600), rat (P09951), mouse (O88935), mammals. Weaker signal: chicken, zebrafish, other vertebrates. Other species not tested yet.
Specificity	Specific for synapsin 1a and 1b independent of phosphorylation state. K.O. validated
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 46.1. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. IHC-G: Fixation with 9% glyoxal, 8% acetic acid, in ddH2O, pH 4.2-4.4, according to Konno et al. 2023 is recommended. ExM: This antibody has been successfully used for the epitope-preserving magnified analysis of the proteome (eMAP) expansion microscopy method (Park et al. 2021. PMID: 34767453). DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).
Data sheet	106_008.pdf

Synapsin1 antibody - 106 008 K.O.

References for Synapsin1 - 106 008