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Synaptotagmin 1 cytoplasmic tail - 105 318

Ca2+-sensor on synaptic vesicles, triggers neurotransmitter release
Monoclonal Recombinant Guinea pig purified IgG
Cat. No.: 105 318
Amount: 50 µg
Price: $415.00
Cat. No. 105 318 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 100 up to 1 : 500 gallery  
Clone Gp41.1
Subtype IgG2 (κ light chain)
Immunogen Recombinant protein corresponding to AA 80 to 421 from rat Synaptotagmin1 (UniProt Id: P21707)
Epitop Epitop: AA 150 to 240 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: rat (P21707), mouse (P46096), human (P21579), mammals, zebrafish.
Other species not tested yet.
Specificity Specific for mammalian synaptotagmin 1, no cross-reactivity to other synaptotagmins K.O.
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 41.1. The constant regions of the heavy and light chains have been replaced by Guinea pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
Since synaptotagmin 1 is unevenly expressed in neuronal subpopulations and may, in fact, be missing from some, it is not well suited as a general marker for synapses.
WB: Non-boiled samples are recommended for SDS-PAGE.

Data sheet 105_318.pdf
Cat. No.: 105 318
Quantity: 50 µg
Price: $415.00
Background
Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.