Synaptotagmin1 antibody cytoplasmic tail - 105 318 K.O.

Synaptotagmin1 is a Ca²⁺-sensor on synaptic vesicles that triggers neurotransmitter release

Guinea pig monoclonal recombinant IgG

Cat. No.: 105 318

Amount: 50 µg

Price: $415.00

Cat. No. 105 318	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 100 up to 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Gp41.1
Subtype	IgG2 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 80 to 421 from rat Synaptotagmin1 (UniProt Id: P21707)
Epitop	AA 150 to 240 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity	Reacts with: rat (P21707), mouse (P46096), human (P21579), mammals, zebrafish. Other species not tested yet.
Specificity	K.O. validated
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 41.1. The constant regions of the heavy and light chains have been replaced by Guinea pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells. Since synaptotagmin 1 is unevenly expressed in neuronal subpopulations and may, in fact, be missing from some, it is not well suited as a general marker for synapses. WB: Non-boiled samples are recommended for SDS-PAGE.
Data sheet	105_318.pdf

Cat. No.: 105 318

Amount: 50 µg

Price: $415.00

ICC

IHC

IHC-P

Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca²⁺-binding C2-domains.
Ca²⁺-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca²⁺-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.

1 ) Geppert M et al., Annu. Rev. Neurosci. (1998)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Südhof TC et al., Nature (1995)
4 ) Geppert M et al., Cell (1994)
5 ) Brose N et al., Science (1992)
6 ) Perin MS et al., Nature (1990)

Protocols

ICC

IHC

IHC-P

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