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Home > Products > 105 311AT647N

Synaptotagmin1 antibody luminal domain - 105 311AT647N

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Mouse monoclonal purified IgG
Cat. No.: 105 311AT647N
Amount: 100 µg
Price: $470.00
Cat. No. 105 311AT647N 100 µg purified IgG, lyophilized, fluorescence-labeled with ATTO® 647N.
Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 50 up to 1 : 300 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
Label ATTO 647N
Clone 604.2
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: rat (P21707).
No signal: mouse (P46096), zebrafish.
Other species not tested yet.
Remarks

ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.
Data sheet 105_311at647n.pdf

References for Synaptotagmin1 - 105 311AT647N

Presynaptic activity and protein turnover are correlated at the single-synapse level.
Jähne S, Mikulasch F, Heuer HGH, Truckenbrodt S, Agüi-Gonzalez P, Grewe K, Vogts A, Rizzoli SO, Priesemann V
Cell reports (2021) 3411: 108841. 105 311AT647N ICC; tested species: rat
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 105 311AT647N ICC, UPTAKE; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 105 311AT647N ICC; tested species: rat
Blocking endocytosis enhances short-term synaptic depression under conditions of normal availability of vesicles.
Hua Y, Woehler A, Kahms M, Haucke V, Neher E, Klingauf J
Neuron (2013) 802: 343-9. 105 311AT647N ICC; tested species: rat
Limited intermixing of synaptic vesicle components upon vesicle recycling.
Opazo F, Punge A, Bückers J, Hoopmann P, Kastrup L, Hell SW, Rizzoli SO
Traffic (Copenhagen, Denmark) (2010) 116: 800-12. 105 311AT647N ICC
Cat. No.: 105 311AT647N
Amount: 100 µg
Price: $470.00
ICC
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