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Synaptotagmin 1 lumenal domain - 105 311AF

Ca2+-sensor on synaptic vesicles, triggers neurotransmitter release
Monoclonal mouse purified IgG
Cat. No.: 105 311AF
Amount: 100 µg
Price: 465.00 €
Cat. No. 105 311AF 100 µg purified IgG, lyophilized, fluorescence-labeled with AcidiFluor Orange 

AcidiFluor Orange® is a pH-sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the cellular compartment e.g. the synaptic vesicle.
AcidiFluor Orange can be detected with the filter sets for Cy3.

CyDyes, Cy2, Cy3, and Cy5 are trademarks of GE-Healthcare Ltd.

This product or portions thereof is manufactured under license from Tokyo University and is covered by Patents Application Number US 2010068733, US 20140248654, EP2098529A1/B1, JP WO 2013035767A, JP 5636080B2, WO 2008/0599910A1, WO 2016/1579937A1 and issued patents US 8258171 and US 9784732 and related patents. Acidifluor®, and Acidifluor Orange® and related products are trademarks of Goryo Chemical. Purchase of reagents related to the Acidifluor Series technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from: Goryo Chemical, EAREE Bldg., SF Kita 8 Nishi 18-35-100, Chuo-Ku, Sapporo, 060-0008 Japan.

. Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
EM: N/A
ELISA: N/A
FACS: not tested yet
Label AcidiFluor Orange
Clone 604.2
Subtype IgG1
Immunogen Synthetic peptide corresponding to AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707)
Epitop Epitop: AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: rat (P21707).
No signal: mouse (P46096), zebrafish.
Other species not tested yet.
Remarks

This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures. The pH sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen.

Data sheet 105_311af.pdf
Cat. No.: 105 311AF
Quantity: 100 µg
Price: 465.00 €
Background
Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.
Protocols