Cat. No.: 105 311AF
Amount: 100 µg
Price:
$470.00
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pH-sensitive probe fluorescent only under acidic conditions
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| Cat. No. 105 311AF |
100 µg purified IgG, lyophilized, fluorescence-labeled with AcidiFluor Orange. Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications |
IP: N/A ICC: 1 : 100 up to 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet EM: N/A ELISA: N/A FACS: not tested yet |
| Label | AcidiFluor Orange |
| Clone | 604.2 |
| Subtype | IgG1 |
| Immunogen | Synthetic peptide corresponding to residues near the amino terminus of rat Synaptotagmin1 (UniProt Id: P21707) |
| Reactivity |
Reacts with: rat (P21707). No signal: mouse (P46096), zebrafish. Other species not tested yet. |
| Remarks |
ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures. The pH sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen. |
| Data sheet | 105_311af.pdf |
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