Synaptic Systems - Synaptotagmin2

Cat. No.: 105 223

Amount: 50 µg

Price: $465.00

Cat. No. 105 223	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 100 (see remarks) gallery IHC: 1 : 500 gallery IHC-P: not tested yet
Immunogen	Synthetic peptide corresponding to AA 1 to 11 from mouse Synaptotagmin2 (UniProt Id: P46097)
Reactivity	Reacts with: rat (P29101), mouse (P46097). Other species not tested yet.
Remarks	ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.
Data sheet	105_223.pdf

References for Synaptotagmin2 - 105 223

ICC (1)
IHC (8)

Astrocyte-secreted neurocan controls inhibitory synapse formation and function.
Irala D, Wang S, Sakers K, Nagendren L, Ulloa Severino FP, Bindu DS, Savage JT, Eroglu C
Neuron (2024) 11210: 1657-1675.e10. 105 223 ICC, IHC; tested species: mouse,rat

The proteomic landscape of synaptic diversity across brain regions and cell types.
van Oostrum M, Blok TM, Giandomenico SL, Tom Dieck S, Tushev G, Fürst N, Langer JD, Schuman EM
Cell (2023) 18624: 5411-5427.e23. 105 223 IHC; tested species: mouse

The complement inhibitor CD59 is required for GABAergic synaptic transmission in the dentate gyrus.
Wen L, Yang X, Wu Z, Fu S, Zhan Y, Chen Z, Bi D, Shen Y
Cell reports (2023) 424: 112349. 105 223 IHC; tested species: mouse

Chronic partial TrkB activation reduces seizures and mortality in a mouse model of Dravet syndrome.
Gu F, Parada I, Yang T, Longo FM, Prince DA
Proceedings of the National Academy of Sciences of the United States of America (2022) 1197: . 105 223 IHC; tested species: mouse

Myelination synchronizes cortical oscillations by consolidating parvalbumin-mediated phasic inhibition.
Dubey M, Pascual-Garcia M, Helmes K, Wever DD, Hamada MS, Kushner SA, Kole MHP
eLife (2022) 11: . 105 223 IHC; tested species: mouse

ROCK/PKA inhibition rescues hippocampal hyperexcitability and GABAergic neuron alterations in Oligophrenin-1 Knock-out mouse model of X-linked intellectual disability.
Busti I, Allegra M, Spalletti C, Panzi C, Restani L, Billuart P, Caleo M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 105 223 IHC; tested species: mouse

Inositol polyphosphate multikinase deficiency leads to aberrant induction of synaptotagmin-2 in the forebrain.
Park J, Park SJ, Kim S
Molecular brain (2019) 121: 58. 105 223 IHC; tested species: mouse

Control of exocytosis by synaptotagmins and otoferlin in auditory hair cells.
Beurg M, Michalski N, Safieddine S, Bouleau Y, Schneggenburger R, Chapman ER, Petit C, Dulon D
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13281-90. 105 223 IHC; tested species: rat

Cat. No.: 105 223

Amount: 50 µg

Price: $465.00

References
ICC (1)
IHC (8)

ICC

IHC

Background

Synaptotagmin2 is an integral membrane glycoprotein of neuronal synaptic vesicles. It is very similar to synaptotagmin1 but shows a partly complementary expression pattern in the CNS. Synaptotagmin2 lacks a CAMK II/PKC phosphorylation site which is present in synaptotagmin1.
Recently synaptotagmin2 has been shown to be an alternative Ca²⁺ sensor for fast secretion.

1 ) Pang ZP et al., J. Neurosci. (2006)
2 ) Pang ZP et al., EMBO J. (2006)
3 ) Lee BH et al., Mol. Cell (2004)
4 ) Lindmark IM et al., Biochim. Biophys. Acta (2002)
5 ) Kida Y et al., FEBS Lett. (2001)
6 ) Baram D et al., J. Exp. Med. (1999)
7 ) Geppert M et al., J. Biol. Chem. (1991)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

Standard protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

Other products for this target

Synaptotagmin2 antibody luminal domain - 105 223

References for Synaptotagmin2 - 105 223