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Home > Products > 105 221

Synaptotagmin1 antibody luminal domain - 105 221 K.O.

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Mouse monoclonal hybridoma supernatant
Cat. No.: 105 221
Amount: 200 µl
Price: $365.00
Cat. No. 105 221 200 µl hybridoma supernatant, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 10000 (AP staining) (see remarks)
IP: yes
ICC: 1 : 100 (see remarks) gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
Clone 604.1
Subtype IgG3 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: rat (P21707), mouse (P46096).
No signal: zebrafish.
Other species not tested yet.
Specificity K.O. validated PubMed: 26195798
Remarks

WB: Only detects rat Synaptotagmin1 in westernblots.
ICC: Antibody is suitable for the labeling of recycling vesicles of living rat and mouse neurons. It detects PFA fixed rat Synaptotagmin1, but is negative on PFA fixed mouse Synaptotagmin1.
IHC: Antibody detects PFA fixed rat Synaptotagmin1, but is negative on PFA fixed mouse Synaptotagmin1.
Data sheet 105_221.pdf

References for Synaptotagmin1 - 105 221

Structural elements that underlie Doc2β function during asynchronous synaptic transmission.
Xue R, Gaffaney JD, Chapman ER
Proceedings of the National Academy of Sciences of the United States of America (2015) 11231: E4316-25. 105 221 WB, ICC; KO verified; tested species: mouse
CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 105 221 WB; tested species: mouse
SV2B regulates synaptotagmin 1 by direct interaction.
Lazzell DR, Belizaire R, Thakur P, Sherry DM, Janz R
The Journal of biological chemistry (2004) 27950: 52124-31. 105 221 IP, WB; tested species: mouse
Calcium-dependent interaction of the cytoplasmic region of synaptotagmin with membranes. Autonomous function of a single C2-homologous domain.
Chapman ER, Jahn R
The Journal of biological chemistry (1994) 2698: 5735-41. 105 221 WB; tested species: rat
Cat. No.: 105 221
Amount: 200 µl
Price: $365.00
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