Synaptic Systems - Synaptotagmin1

Cat. No.: 105 221

Amount: 200 µl

Price: $360.00

Cat. No. 105 221	200 µl hybridoma supernatant, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) (see remarks) IP: yes ICC: 1 : 100 (see remarks) gallery IHC: 1 : 500 (see remarks) gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	604.1
Subtype	IgG3 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity	Reacts with: rat (P21707), mouse (P46096). No signal: zebrafish. Other species not tested yet.
Specificity	K.O. validated PubMed: 26195798
Remarks	WB: Only detects rat Synaptotagmin1 in westernblots. ICC: Antibody is suitable for the labeling of recycling vesicles of living rat and mouse neurons. It detects PFA fixed rat Synaptotagmin1, but is negative on PFA fixed mouse Synaptotagmin1. IHC: Antibody detects PFA fixed rat Synaptotagmin1, but is negative on PFA fixed mouse Synaptotagmin1.
Data sheet	105_221.pdf

References for Synaptotagmin1 - 105 221

Structural elements that underlie Doc2β function during asynchronous synaptic transmission.
Xue R, Gaffaney JD, Chapman ER
Proceedings of the National Academy of Sciences of the United States of America (2015) 11231: E4316-25. 105 221 WB, ICC; KO verified; tested species: mouse

CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 105 221 WB; tested species: mouse

SV2B regulates synaptotagmin 1 by direct interaction.
Lazzell DR, Belizaire R, Thakur P, Sherry DM, Janz R
The Journal of biological chemistry (2004) 27950: 52124-31. 105 221 IP, WB; tested species: mouse

Calcium-dependent interaction of the cytoplasmic region of synaptotagmin with membranes. Autonomous function of a single C2-homologous domain.
Chapman ER, Jahn R
The Journal of biological chemistry (1994) 2698: 5735-41. 105 221 WB; tested species: rat

Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission.
Martineau M, Shi T, Puyal J, Knolhoff AM, Dulong J, Gasnier B, Klingauf J, Sweedler JV, Jahn R, Mothet JP
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 338: 3413-23. 105 221 IP, IHC; tested species: rat

BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons.
Shinoda Y, Ahmed S, Ramachandran B, Bharat V, Brockelt D, Altas B, Dean C
Frontiers in synaptic neuroscience (2014) 6: 27. 105 221 ICC; tested species: rat

Imaging of evoked dense-core-vesicle exocytosis in hippocampal neurons reveals long latencies and kiss-and-run fusion events.
Xia X, Lessmann V, Martin TF
Journal of cell science (2009) 122Pt 1: 75-82. 105 221 ICC; tested species: rat

Disease-linked mutations in Munc18-1 deplete synaptic Doc2.
Guiberson NGL, Black LS, Haller JE, Brukner A, Abramov D, Ahmad S, Xie YX, Sharma M, Burré J
Brain : a journal of neurology (2024) : . 105 221 UPTAKE; tested species: mouse

Targeted stabilization of Munc18-1 function via pharmacological chaperones.
Abramov D, Guiberson NGL, Daab A, Na Y, Petsko GA, Sharma M, Burré J
EMBO molecular medicine (2020) : e12354. 105 221 UPTAKE; tested species: mouse

Mechanism-based rescue of Munc18-1 dysfunction in varied encephalopathies by chemical chaperones.
Guiberson NGL, Pineda A, Abramov D, Kharel P, Carnazza KE, Wragg RT, Dittman JS, Burré J
Nature communications (2018) 91: 3986. 105 221 UPTAKE; tested species: mouse

Loss of Doc2-Dependent Spontaneous Neurotransmission Augments Glutamatergic Synaptic Strength.
Ramirez DMO, Crawford DC, Chanaday NL, Trauterman B, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3726: 6224-6230. 105 221 UPTAKE; tested species: rat

Postsynaptic Neuroligin1 regulates presynaptic maturation.
Wittenmayer N, Körber C, Liu H, Kremer T, Varoqueaux F, Chapman ER, Brose N, Kuner T, Dresbach T
Proceedings of the National Academy of Sciences of the United States of America (2009) 10632: 13564-9. 105 221 UPTAKE; tested species: mouse

STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis.
Willig KI, Rizzoli SO, Westphal V, Jahn R, Hell SW
Nature (2006) 4407086: 935-9. 105 221 UPTAKE

Borna disease virus blocks potentiation of presynaptic activity through inhibition of protein kinase C signaling.
Volmer R, Monnet C, Gonzalez-Dunia D
PLoS pathogens (2006) 23: e19. 105 221 UPTAKE; tested species: rat

Phosphorylation of synapsin I by cAMP-dependent protein kinase controls synaptic vesicle dynamics in developing neurons.
Bonanomi D, Menegon A, Miccio A, Ferrari G, Corradi A, Kao HT, Benfenati F, Valtorta F
The Journal of neuroscience : the official journal of the Society for Neuroscience (2005) 2532: 7299-308. 105 221 UPTAKE

Synaptic targeting of N-type calcium channels in hippocampal neurons.
Maximov A, Bezprozvanny I
The Journal of neuroscience : the official journal of the Society for Neuroscience (2002) 2216: 6939-52. 105 221 UPTAKE; tested species: rat

Cat. No.: 105 221

Amount: 200 µl

Price: $360.00

STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis.
Willig KI, Rizzoli SO, Westphal V, Jahn R, Hell SW
Nature (2006) 4407086: 935-9. 105 221 UPTAKE

ICC

IHC

Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca²⁺-binding C2-domains.
Ca²⁺-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca²⁺-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.

1 ) Geppert M et al., Annu. Rev. Neurosci. (1998)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Südhof TC et al., Nature (1995)
4 ) Geppert M et al., Cell (1994)
5 ) Brose N et al., Science (1992)
6 ) Perin MS et al., Nature (1990)

Protocols

Standard protocol

ICC

IHC

Other products for this target

Synaptotagmin1 antibody luminal domain - 105 221 K.O.

References for Synaptotagmin1 - 105 221