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Home > Products > 105 221

Synaptotagmin1 antibody luminal domain - 105 221 K.O.

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Mouse monoclonal hybridoma supernatant
Cat. No.: 105 221
Amount: 200 µl
Price: $360.00
Cat. No. 105 221 200 µl hybridoma supernatant, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 10000 (AP staining) (see remarks)
IP: yes
ICC: 1 : 100 (see remarks) gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
Clone 604.1
Subtype IgG3 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: rat (P21707), mouse (P46096).
No signal: zebrafish.
Other species not tested yet.
Specificity K.O. validated PubMed: 26195798
Remarks

WB: Only detects rat Synaptotagmin1 in westernblots.
ICC: Antibody is suitable for the labeling of recycling vesicles of living rat and mouse neurons. It detects PFA fixed rat Synaptotagmin1, but is negative on PFA fixed mouse Synaptotagmin1.
IHC: Antibody detects PFA fixed rat Synaptotagmin1, but is negative on PFA fixed mouse Synaptotagmin1.
Data sheet 105_221.pdf

References for Synaptotagmin1 - 105 221

Structural elements that underlie Doc2β function during asynchronous synaptic transmission.
Xue R, Gaffaney JD, Chapman ER
Proceedings of the National Academy of Sciences of the United States of America (2015) 11231: E4316-25. 105 221 WB, ICC; KO verified; tested species: mouse
CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 105 221 WB; tested species: mouse
SV2B regulates synaptotagmin 1 by direct interaction.
Lazzell DR, Belizaire R, Thakur P, Sherry DM, Janz R
The Journal of biological chemistry (2004) 27950: 52124-31. 105 221 IP, WB; tested species: mouse
Calcium-dependent interaction of the cytoplasmic region of synaptotagmin with membranes. Autonomous function of a single C2-homologous domain.
Chapman ER, Jahn R
The Journal of biological chemistry (1994) 2698: 5735-41. 105 221 WB; tested species: rat
Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission.
Martineau M, Shi T, Puyal J, Knolhoff AM, Dulong J, Gasnier B, Klingauf J, Sweedler JV, Jahn R, Mothet JP
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 338: 3413-23. 105 221 IP, IHC; tested species: rat
SV2B regulates synaptotagmin 1 by direct interaction.
Lazzell DR, Belizaire R, Thakur P, Sherry DM, Janz R
The Journal of biological chemistry (2004) 27950: 52124-31. 105 221 IP, WB; tested species: mouse
Structural elements that underlie Doc2β function during asynchronous synaptic transmission.
Xue R, Gaffaney JD, Chapman ER
Proceedings of the National Academy of Sciences of the United States of America (2015) 11231: E4316-25. 105 221 WB, ICC; KO verified; tested species: mouse
BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons.
Shinoda Y, Ahmed S, Ramachandran B, Bharat V, Brockelt D, Altas B, Dean C
Frontiers in synaptic neuroscience (2014) 6: 27. 105 221 ICC; tested species: rat
Imaging of evoked dense-core-vesicle exocytosis in hippocampal neurons reveals long latencies and kiss-and-run fusion events.
Xia X, Lessmann V, Martin TF
Journal of cell science (2009) 122Pt 1: 75-82. 105 221 ICC; tested species: rat
Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission.
Martineau M, Shi T, Puyal J, Knolhoff AM, Dulong J, Gasnier B, Klingauf J, Sweedler JV, Jahn R, Mothet JP
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 338: 3413-23. 105 221 IP, IHC; tested species: rat
Targeted stabilization of Munc18-1 function via pharmacological chaperones.
Abramov D, Guiberson NGL, Daab A, Na Y, Petsko GA, Sharma M, Burré J
EMBO molecular medicine (2020) : e12354. 105 221 UPTAKE; tested species: mouse
Mechanism-based rescue of Munc18-1 dysfunction in varied encephalopathies by chemical chaperones.
Guiberson NGL, Pineda A, Abramov D, Kharel P, Carnazza KE, Wragg RT, Dittman JS, Burré J
Nature communications (2018) 91: 3986. 105 221 UPTAKE; tested species: mouse
Loss of Doc2-Dependent Spontaneous Neurotransmission Augments Glutamatergic Synaptic Strength.
Ramirez DMO, Crawford DC, Chanaday NL, Trauterman B, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3726: 6224-6230. 105 221 UPTAKE; tested species: rat
Postsynaptic Neuroligin1 regulates presynaptic maturation.
Wittenmayer N, Körber C, Liu H, Kremer T, Varoqueaux F, Chapman ER, Brose N, Kuner T, Dresbach T
Proceedings of the National Academy of Sciences of the United States of America (2009) 10632: 13564-9. 105 221 UPTAKE; tested species: mouse
STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis.
Willig KI, Rizzoli SO, Westphal V, Jahn R, Hell SW
Nature (2006) 4407086: 935-9. 105 221 UPTAKE
Borna disease virus blocks potentiation of presynaptic activity through inhibition of protein kinase C signaling.
Volmer R, Monnet C, Gonzalez-Dunia D
PLoS pathogens (2006) 23: e19. 105 221 UPTAKE; tested species: rat
Phosphorylation of synapsin I by cAMP-dependent protein kinase controls synaptic vesicle dynamics in developing neurons.
Bonanomi D, Menegon A, Miccio A, Ferrari G, Corradi A, Kao HT, Benfenati F, Valtorta F
The Journal of neuroscience : the official journal of the Society for Neuroscience (2005) 2532: 7299-308. 105 221 UPTAKE
Synaptic targeting of N-type calcium channels in hippocampal neurons.
Maximov A, Bezprozvanny I
The Journal of neuroscience : the official journal of the Society for Neuroscience (2002) 2216: 6939-52. 105 221 UPTAKE; tested species: rat
Cat. No.: 105 221
Amount: 200 µl
Price: $360.00
Structural elements that underlie Doc2β function during asynchronous synaptic transmission.
Xue R, Gaffaney JD, Chapman ER
Proceedings of the National Academy of Sciences of the United States of America (2015) 11231: E4316-25. 105 221 WB, ICC; KO verified; tested species: mouse
CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 105 221 WB; tested species: mouse
SV2B regulates synaptotagmin 1 by direct interaction.
Lazzell DR, Belizaire R, Thakur P, Sherry DM, Janz R
The Journal of biological chemistry (2004) 27950: 52124-31. 105 221 IP, WB; tested species: mouse
Calcium-dependent interaction of the cytoplasmic region of synaptotagmin with membranes. Autonomous function of a single C2-homologous domain.
Chapman ER, Jahn R
The Journal of biological chemistry (1994) 2698: 5735-41. 105 221 WB; tested species: rat
Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission.
Martineau M, Shi T, Puyal J, Knolhoff AM, Dulong J, Gasnier B, Klingauf J, Sweedler JV, Jahn R, Mothet JP
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 338: 3413-23. 105 221 IP, IHC; tested species: rat
SV2B regulates synaptotagmin 1 by direct interaction.
Lazzell DR, Belizaire R, Thakur P, Sherry DM, Janz R
The Journal of biological chemistry (2004) 27950: 52124-31. 105 221 IP, WB; tested species: mouse
Structural elements that underlie Doc2β function during asynchronous synaptic transmission.
Xue R, Gaffaney JD, Chapman ER
Proceedings of the National Academy of Sciences of the United States of America (2015) 11231: E4316-25. 105 221 WB, ICC; KO verified; tested species: mouse
BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons.
Shinoda Y, Ahmed S, Ramachandran B, Bharat V, Brockelt D, Altas B, Dean C
Frontiers in synaptic neuroscience (2014) 6: 27. 105 221 ICC; tested species: rat
Imaging of evoked dense-core-vesicle exocytosis in hippocampal neurons reveals long latencies and kiss-and-run fusion events.
Xia X, Lessmann V, Martin TF
Journal of cell science (2009) 122Pt 1: 75-82. 105 221 ICC; tested species: rat
Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission.
Martineau M, Shi T, Puyal J, Knolhoff AM, Dulong J, Gasnier B, Klingauf J, Sweedler JV, Jahn R, Mothet JP
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 338: 3413-23. 105 221 IP, IHC; tested species: rat
Targeted stabilization of Munc18-1 function via pharmacological chaperones.
Abramov D, Guiberson NGL, Daab A, Na Y, Petsko GA, Sharma M, Burré J
EMBO molecular medicine (2020) : e12354. 105 221 UPTAKE; tested species: mouse
Mechanism-based rescue of Munc18-1 dysfunction in varied encephalopathies by chemical chaperones.
Guiberson NGL, Pineda A, Abramov D, Kharel P, Carnazza KE, Wragg RT, Dittman JS, Burré J
Nature communications (2018) 91: 3986. 105 221 UPTAKE; tested species: mouse
Loss of Doc2-Dependent Spontaneous Neurotransmission Augments Glutamatergic Synaptic Strength.
Ramirez DMO, Crawford DC, Chanaday NL, Trauterman B, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3726: 6224-6230. 105 221 UPTAKE; tested species: rat
Postsynaptic Neuroligin1 regulates presynaptic maturation.
Wittenmayer N, Körber C, Liu H, Kremer T, Varoqueaux F, Chapman ER, Brose N, Kuner T, Dresbach T
Proceedings of the National Academy of Sciences of the United States of America (2009) 10632: 13564-9. 105 221 UPTAKE; tested species: mouse
STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis.
Willig KI, Rizzoli SO, Westphal V, Jahn R, Hell SW
Nature (2006) 4407086: 935-9. 105 221 UPTAKE
Borna disease virus blocks potentiation of presynaptic activity through inhibition of protein kinase C signaling.
Volmer R, Monnet C, Gonzalez-Dunia D
PLoS pathogens (2006) 23: e19. 105 221 UPTAKE; tested species: rat
Phosphorylation of synapsin I by cAMP-dependent protein kinase controls synaptic vesicle dynamics in developing neurons.
Bonanomi D, Menegon A, Miccio A, Ferrari G, Corradi A, Kao HT, Benfenati F, Valtorta F
The Journal of neuroscience : the official journal of the Society for Neuroscience (2005) 2532: 7299-308. 105 221 UPTAKE
Synaptic targeting of N-type calcium channels in hippocampal neurons.
Maximov A, Bezprozvanny I
The Journal of neuroscience : the official journal of the Society for Neuroscience (2002) 2216: 6939-52. 105 221 UPTAKE; tested species: rat
ICC
ICC
IHC
Background
Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.
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