Synaptic Systems - Synaptotagmin5/9

Cat. No.: 105 053

Amount: 50 µg

Price: $375.00

Cat. No. 105 053	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 50 to 108 from mouse Synaptotagmin5 (UniProt Id: Q9R0N5) (UniProt Id: Q9R0N5)
Reactivity	Reacts with: rat (P47861), mouse (Q9R0N5). Other species not tested yet.
Specificity	Specific for synaptotagmin 5 (UniProt Id: Q9R0N5) sometimes referred to as synaptotagmin 9. Does not detect (UniProt Id: Q9R0N9); K.O. validated PubMed: 37432648
Data sheet	105_053.pdf

References for Synaptotagmin5/9 - 105 053

Palmitoylation couples insulin hypersecretion with β cell failure in diabetes.
Dong G, Adak S, Spyropoulos G, Zhang Q, Feng C, Yin L, Speck SL, Shyr Z, Morikawa S, Kitamura RA, Kathayat RS, et al.
Cell metabolism (2023) : . 105 053 WB; tested species: rat

Genetic ablation of synaptotagmin-9 alters tomosyn-1 function to increase insulin secretion from pancreatic β-cells improving glucose clearance.
Rahman MM, Pathak A, Schueler KL, Alsharif H, Michl A, Alexander J, Kim JA, Bhatnagar S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2023) 378: e23075. 105 053 WB, ICC; KO verified; tested species: mouse

Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in Striatal Neurons by Regulating Substance P Secretion.
Seibert MJ, Evans CS, Stanley KS, Wu Z, Chapman ER
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 439: 1475-1491. 105 053 WB, ICC; KO verified; tested species: mouse

Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis.
Bello OD, Jouannot O, Chaudhuri A, Stroeva E, Coleman J, Volynski KE, Rothman JE, Krishnakumar SS
Proceedings of the National Academy of Sciences of the United States of America (2018) 11532: E7624-E7631. 105 053 WB; tested species: rat

A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 105 053 WB

Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen.
Dean C, Dunning FM, Liu H, Bomba-Warczak E, Martens H, Bharat V, Ahmed S, Chapman ER
Molecular biology of the cell (2012) 239: 1715-27. 105 053 WB, ICC

Sex-specific regulation of follicle-stimulating hormone secretion by synaptotagmin 9.
Roper LK, Briguglio JS, Evans CS, Jackson MB, Chapman ER
Nature communications (2015) 6: 8645. 105 053 ICC

GABAergic-like dopamine synapses in the brain.
Kim HJ, Hwang B, Reva M, Lee J, Lee BE, Lee Y, Cho EJ, Jeong M, Lee SE, Myung K, Baik JH, et al.
Cell reports (2023) 4210: 113239. 105 053 IHC; tested species: mouse

Cat. No.: 105 053

Amount: 50 µg

Price: $375.00

Sex-specific regulation of follicle-stimulating hormone secretion by synaptotagmin 9.
Roper LK, Briguglio JS, Evans CS, Jackson MB, Chapman ER
Nature communications (2015) 6: 8645. 105 053 ICC

ICC

IHC

IHC-P

Background

Up to now at least 17 synaptotagmins have been identified. Two different synaptotagmins were independently termed synaptotagmin5 by two research groups (see Li et al., 1995 and Craxton & Goeder, 1995). The groups refer to the respective other protein as synaptotagmin9. The synaptotagmin described by Craxton & Goeder is expressed in different tissues like kidney, heart, lung and adipose tissue but shows its highest expression level in brain, where it predominantly locates to an enriched synaptic vesicle fraction. Like other synaptotagmins it contains one transmembrane and two cytosolic C2 domains.

1 ) Li C et al., Nature (1995)
2 ) Iezzi M et al., J. Cell. Sci. (2004)
3 ) Saegusa C et al., J. Biol. Chem. (2002)
4 ) Craxton M et al., FEBS Lett. (1999)
5 ) Hudson AW et al., Proc. Natl. Acad. Sci. U.S.A. (1995)
6 ) Craxton M et al., FEBS Lett. (1995)

Protocols

ICC

IHC

IHC-P

Synaptotagmin5/9 antibody - 105 053 K.O.

References for Synaptotagmin5/9 - 105 053