Tailor-made Antibodies
and Tools for Life Science

VAMP1/2/3 antibody - 104 011

Vamp1, 2 and 3 are major vesicle protein involved in fusion
Mouse monoclonal purified IgG
Cat. No.: 104 011
Amount: 100 µg
Price: $420.00
Cat. No. 104 011 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 100 up to 1 : 500 gallery  
IHC-P: 1 : 500 up to 1 : 1000 gallery  
Clone 10.1
Subtype IgG1
Immunogen Recombinant protein corresponding to AA 1 to 118 from rat Synaptobrevin1 (UniProt Id: Q63666)
Epitop AA 31 to 98 from rat Synaptobrevin1 (UniProt Id: Q63666)
Reactivity Reacts with: human (P23763, P63027, Q15836), rat (Q63666, P63045, P63025), mouse (Q62442, P63044, P63024), vertebrates, invertebrates.
Other species not tested yet.
Specificity Detects the three isoforms VAMP 1, 2, 3.
Remarks

This antibody is not recommended for non-neuronal cells. Please use cat. no. 104 102 for such experiments.
This antibody reacts with the conserved middle-portion of the molecule. Its affinity/avidity is not very high, thus requiring extended incubation periods and sensitive detection systems.
WB: This antibody recognizes the Botulinumtoxin B cleavage product (aa 1 - 76) with reduced affinity. The sensitivity is sufficient for the detection of cleaved recombinant protein. For analysis of toxin treated tissue homogenates cat. no. 104 203 is recommended.

Data sheet 104_011.pdf

References for VAMP1/2/3 - 104 011

Maternal stress programs a demasculinization of glutamatergic transmission in stress-related brain regions of aged rats.
Verhaeghe R, Gao V, Morley-Fletcher S, Bouwalerh H, Van Camp G, Cisani F, Nicoletti F, Maccari S
GeroScience (2021) : . 104 011 WB; tested species: rat
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 104 011 WB, ICC
A common mechanism for the regulation of vesicular SNAREs on phospholipid membranes.
Hu K, Rickman C, Carroll J, Davletov B
The Biochemical journal (2004) 377Pt 3: 781-5. 104 011 WB
A role for Sec1/Munc18 proteins in platelet exocytosis.
Schraw TD, Lemons PP, Dean WL, Whiteheart SW
The Biochemical journal (2003) 374Pt 1: 207-17. 104 011 WB; tested species: human
Cat. No.: 104 011
Amount: 100 µg
Price: $420.00