Cat. No.: 104 011
Amount: 100 µg
Price:
$415.00
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| Cat. No. 104 011 |
100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Clone | 10.1 |
| Subtype | IgG1 |
| Immunogen | Recombinant protein corresponding to AA 1 to 118 from rat Synaptobrevin1 (UniProt Id: Q63666) |
| Epitop |
AA 31 to 98 from rat Synaptobrevin1 (UniProt Id: Q63666) |
| Reactivity |
Reacts with: human (P23763, P63027, Q15836), rat (Q63666, P63045, P63025), mouse (Q62442, P63044, P63024), vertebrates, invertebrates. Other species not tested yet. |
| Specificity | Detects the three isoforms VAMP 1, 2, 3. |
| Remarks |
This antibody is not recommended for non-neuronal cells. Please use cat. no. 104 102 for such experiments. |
| Data sheet | 104_011.pdf |
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Synaptobrevins/VAMPs represents a family of integral membrane proteins of 11-13 kDa with the N-terminal region exposed to the cytoplasm and a C-terminal transmembrane domain. Two isoforms were identified in the mammalian CNS, synaptobrevin 1 (VAMP 1 or p18-1) and synaptobrevin 2 (VAMP 2 or p18-2) that differ in their distribution within different brain regions.
Synaptobrevin 1 is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin 2 are present in many other tissues where the protein resides on specialized microvesicles.
In non-neuronal cells the third isoform, cellubrevin (VAMP 3), is present where it is localized to an endosomal membrane pool.
Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v-SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule.