|Cat. No. 102 008||50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (AP staining) gallery
IP: not tested yet
ICC: 1 : 500 up to 1 : 2000 gallery
IHC: 1 : 500 up to 1 : 2000 (see remarks) gallery
IHC-P/FFPE: 1 : 1000 up to 1 : 4000 gallery
|Subtype||IgG1 (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 250 to 263 from rat Synaptoporin (UniProt Id: P22831)|
Epitop: AA 250 to 263 from rat Synaptoporin (UniProt Id: P22831)
Reacts with: mouse (Q8BGN8), rat (P22831).
Other species not tested yet.
|Specificity||Specific for synaptoporin|
|Matching control protein/peptide||102-1P|
This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 44E3D12. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Synaptoporin, also known as synaptophysin 2 and p38-2, is highly homologous to synaptophysin 1 but encoded by a different gene. Like synaptopysin 1, synaptoporin contains four transmembrane regions and a short cytoplasmic tail. Unlike synaptophysin 1, it is not glycosylated.
The distributions of synaptophysin 1 and synaptoporin are different. Synaptophysin 1 is more uniformly expressed whereas synaptoporin is particularly enriched in mossy fiber synapses in the hippocampus. It is thus an excellent marker for subsets of synapses.