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Synaptoporin - 102 008

A synaptic vesicle membrane protein enriched in Mossy fibres
Monoclonal Recombinant rabbit purified IgG
Cat. No.: 102 008
Amount: 50 µg
Price: $415.00
Cat. No. 102 008 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 2000 gallery  
IHC: 1 : 500 up to 1 : 2000 (see remarks) gallery  
IHC-P/FFPE: 1 : 1000 up to 1 : 4000 gallery  
Clone Rb44E3D12
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 250 to 263 from rat Synaptoporin (UniProt Id: P22831)
Epitop Epitop: AA 250 to 263 from rat Synaptoporin (UniProt Id: P22831)
Reactivity Reacts with: mouse (Q8BGN8), rat (P22831).
Other species not tested yet.
Specificity Specific for synaptoporin
Matching control protein/peptide 102-1P

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 44E3D12. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC: In addition to the expected strong mossy fibre staining, this antibody shows some weak unspecific staining of cell bodies in some brain regions.

Data sheet 102_008.pdf
Cat. No.: 102 008
Quantity: 50 µg
Price: $415.00

Synaptoporin, also known as synaptophysin 2 and p38-2, is highly homologous to synaptophysin 1 but encoded by a different gene. Like synaptopysin 1, synaptoporin contains four transmembrane regions and a short cytoplasmic tail. Unlike synaptophysin 1, it is not glycosylated.
The distributions of synaptophysin 1 and synaptoporin are different. Synaptophysin 1 is more uniformly expressed whereas synaptoporin is particularly enriched in mossy fiber synapses in the hippocampus. It is thus an excellent marker for subsets of synapses.