|Cat. No. 102 005||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 up to 1 : 5000 (AP staining) (see remarks) gallery
ICC: 1 : 500 up to 1 : 1000 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 500 gallery
|Immunogen||Synthetic peptide corresponding to AA 250 to 265 from rat Synaptoporin (UniProt Id: P22831)|
Reacts with: rat (P22831), mouse (Q8BGN8), hamster.
Other species not tested yet.
|Specificity||Specific for synaptoporin.|
|Matching control protein/peptide||102-0P|
WB: Less powerful compared to the rabbit antibodies (102 002 and 102 003).
Synaptoporin, also known as synaptophysin 2 and p38-2, is highly homologous to synaptophysin 1 but encoded by a different gene. Like synaptopysin 1, synaptoporin contains four transmembrane regions and a short cytoplasmic tail. Unlike synaptophysin 1, it is not glycosylated.
The distributions of synaptophysin 1 and synaptoporin are different. Synaptophysin 1 is more uniformly expressed whereas synaptoporin is particularly enriched in mossy fiber synapses in the hippocampus. It is thus an excellent marker for subsets of synapses.