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Home > Products > 102 003

Synaptoporin antibody - 102 003

Synaptoporin s a synaptic vesicle membrane protein similar to synaptophysin1 enriched in Mossy fibres
Rabbit polyclonal purified antibody
Cat. No.: 102 003
Amount: 50 µg
Price: $460.00
Cat. No. 102 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 250 to 263 from rat Synaptoporin (UniProt Id: P22831)
Reactivity Reacts with: rat (P22831), mouse (Q8BGN8), hamster, human (Q8TBG9).
Other species not tested yet.
Matching control protein/peptide 102-1P
Data sheet 102_003.pdf

References for Synaptoporin - 102 003

Non-canonical function of ADAM10 in presynaptic plasticity.
Bär J, Fanutza T, Reimann CC, Seipold L, Grohe M, Bolter JR, Delfs F, Bucher M, Gee CE, Schweizer M, Saftig P, et al.
Cellular and molecular life sciences : CMLS (2024) 811: 342. 102 003 IHC; tested species: mouse
Midazolam prevents the adverse outcome of neonatal asphyxia.
Welzel B, Schmidt R, Johne M, Löscher W
Annals of neurology (2022) : . 102 003 IHC; tested species: rat
Scopolamine prevents aberrant mossy fiber sprouting and facilitates remission of epilepsy after brain injury.
Meller S, Käufer C, Gailus B, Brandt C, Löscher W
Neurobiology of disease (2021) : 105446. 102 003 IHC; tested species: rat
Layer-Specific Vesicular Glutamate Transporter 1 Immunofluorescence Levels Delineate All Layers of the Human Hippocampus Including the Stratum lucidum.
Woelfle S, Boeckers TM
Frontiers in cellular neuroscience (2021) 15: 789903. 102 003 IHC; tested species: human
Neuronal BIN1 Regulates Presynaptic Neurotransmitter Release and Memory Consolidation.
De Rossi P, Nomura T, Andrew RJ, Masse NY, Sampathkumar V, Musial TF, Sudwarts A, Recupero AJ, Le Metayer T, Hansen MT, Shim HN, et al.
Cell reports (2020) 3010: 3520-3535.e7. 102 003 IHC; tested species: mouse
Ultrastructural Correlates of Presynaptic Functional Heterogeneity in Hippocampal Synapses.
Maus L, Lee C, Altas B, Sertel SM, Weyand K, Rizzoli SO, Rhee J, Brose N, Imig C, Cooper BH
Cell reports (2020) 3011: 3632-3643.e8. 102 003 IHC; tested species: mouse
Ultrastructural Imaging of Activity-Dependent Synaptic Membrane-Trafficking Events in Cultured Brain Slices.
Imig C, López-Murcia FJ, Maus L, García-Plaza IH, Mortensen LS, Schwark M, Schwarze V, Angibaud J, Nägerl UV, Taschenberger H, Brose N, et al.
Neuron (2020) : . 102 003 IHC; tested species: mouse
BACE1 partial deletion induces synaptic plasticity deficit in adult mice.
Lombardo S, Chiacchiaretta M, Tarr A, Kim W, Cao T, Sigal G, Rosahl TW, Xia W, Haydon PG, Kennedy ME, Tesco G, et al.
Scientific reports (2019) 91: 19877. 102 003 IHC; tested species: mouse
Early tissue damage and microstructural reorganization predict disease severity in experimental epilepsy.
Janz P, Schwaderlapp N, Heining K, Häussler U, Korvink JG, von Elverfeldt D, Hennig J, Egert U, LeVan P, Haas CA
eLife (2017) 6: . 102 003 IHC; tested species: human
Cat. No.: 102 003
Amount: 50 µg
Price: $460.00
Non-canonical function of ADAM10 in presynaptic plasticity.
Bär J, Fanutza T, Reimann CC, Seipold L, Grohe M, Bolter JR, Delfs F, Bucher M, Gee CE, Schweizer M, Saftig P, et al.
Cellular and molecular life sciences : CMLS (2024) 811: 342. 102 003 IHC; tested species: mouse
Midazolam prevents the adverse outcome of neonatal asphyxia.
Welzel B, Schmidt R, Johne M, Löscher W
Annals of neurology (2022) : . 102 003 IHC; tested species: rat
Scopolamine prevents aberrant mossy fiber sprouting and facilitates remission of epilepsy after brain injury.
Meller S, Käufer C, Gailus B, Brandt C, Löscher W
Neurobiology of disease (2021) : 105446. 102 003 IHC; tested species: rat
Layer-Specific Vesicular Glutamate Transporter 1 Immunofluorescence Levels Delineate All Layers of the Human Hippocampus Including the Stratum lucidum.
Woelfle S, Boeckers TM
Frontiers in cellular neuroscience (2021) 15: 789903. 102 003 IHC; tested species: human
Neuronal BIN1 Regulates Presynaptic Neurotransmitter Release and Memory Consolidation.
De Rossi P, Nomura T, Andrew RJ, Masse NY, Sampathkumar V, Musial TF, Sudwarts A, Recupero AJ, Le Metayer T, Hansen MT, Shim HN, et al.
Cell reports (2020) 3010: 3520-3535.e7. 102 003 IHC; tested species: mouse
Ultrastructural Correlates of Presynaptic Functional Heterogeneity in Hippocampal Synapses.
Maus L, Lee C, Altas B, Sertel SM, Weyand K, Rizzoli SO, Rhee J, Brose N, Imig C, Cooper BH
Cell reports (2020) 3011: 3632-3643.e8. 102 003 IHC; tested species: mouse
Ultrastructural Imaging of Activity-Dependent Synaptic Membrane-Trafficking Events in Cultured Brain Slices.
Imig C, López-Murcia FJ, Maus L, García-Plaza IH, Mortensen LS, Schwark M, Schwarze V, Angibaud J, Nägerl UV, Taschenberger H, Brose N, et al.
Neuron (2020) : . 102 003 IHC; tested species: mouse
BACE1 partial deletion induces synaptic plasticity deficit in adult mice.
Lombardo S, Chiacchiaretta M, Tarr A, Kim W, Cao T, Sigal G, Rosahl TW, Xia W, Haydon PG, Kennedy ME, Tesco G, et al.
Scientific reports (2019) 91: 19877. 102 003 IHC; tested species: mouse
Early tissue damage and microstructural reorganization predict disease severity in experimental epilepsy.
Janz P, Schwaderlapp N, Heining K, Häussler U, Korvink JG, von Elverfeldt D, Hennig J, Egert U, LeVan P, Haas CA
eLife (2017) 6: . 102 003 IHC; tested species: human
WB
ICC
IHC
IHC-P
Background

Synaptoporin, also known as synaptophysin 2 and p38-2, is highly homologous to synaptophysin 1 but encoded by a different gene. Like synaptopysin 1, synaptoporin contains four transmembrane regions and a short cytoplasmic tail. Unlike synaptophysin 1, it is not glycosylated.
The distributions of synaptophysin 1 and synaptoporin are different. Synaptophysin 1 is more uniformly expressed whereas synaptoporin is particularly enriched in mossy fiber synapses in the hippocampus. It is thus an excellent marker for subsets of synapses.
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