Home|||||||Technical Support|

Synaptophysin 1 - 101 203

Major synaptic vesicle membrane protein; neuroendocrine tumor marker
Polyclonal rabbit purified antibody
Cat. No.: 101 203
Amount: 50 µg
Price: $370.00
Cat. No. 101 203 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 5000 up to 1 : 10000 AP staining gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P/FFPE: 1 : 1000 gallery  
Immunogen Recombinant protein corresponding to AA 229 to 287 from rat Synaptophysin1 (UniProt Id: P07825)
Reactivity Reacts with: mouse (Q62277), rat (P07825).
Other species not tested yet.
Specificity Specific for Synaptophysin 1. No cross-reactivity to Synaptoporin/Synaptophysin 2.
Data sheet 101_203.pdf
Cat. No.: 101 203
Quantity: 50 µg
Price: $370.00
Background

Synaptophysin 1, also referred to as p38-1, is a membrane glycoprotein of synaptic vesicles that is ubiquitously expressed in all neurons and in many endocrine cells. It is currently the most widely used marker for nerve terminals and probably the best marker for the pathologist in differentiating neuroendocrine tumors.
Synaptophysin 1 has four transmembrane domains with both N- and C-terminus facing the cytoplasm. It binds to synaptobrevin 1 and synaptobrevin 2 in detergent extracts but its function has not been elucidated completely. It forms a complex with dynamin at high Ca2+ concentration suggesting an involvement in synaptic vesicle endocytosis. As typical for synaptic vesicle proteins, synaptophysin 1 represents a small protein family with two additonal members, synaptoporin (synaptophysin 2) and panthophysin. Like synaptophysin 1, synaptoporin is widely expressed in neurons and colocalizes with synaptophysin 1 on synaptic vesicles whereas panthophysin is expressed in all tissues.