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Home > Products > 101 011C3

Synaptophysin1 antibody - 101 011C3 K.O.

Synaptophysin is a major synaptic vesicle membrane protein and a useful neuroendocrine tumor marker
Mouse monoclonal purified IgG
Cat. No.: 101 011C3
Amount: 50 µg
Price: $475.00
Cat. No. 101 011C3 50 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 3.

Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

Cyanine 2: λex 492 nm / λem 508 nm
Sulfo-Cyanine 3: λex 554 nm / λem 568 nm
Sulfo-Cyanine 5: λex 649 nm / λem 667 nm

(Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy.
Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates.
Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label Sulfo-Cyanine 3
Clone 7.2
Subtype IgG1 (λ light chain)
Immunogen Full-length recombinant rat synaptophysin (UniProt Id: P07825)
Epitop AA 219 to 307 from rat Synaptophysin1 (UniProt Id: P07825)
Reactivity Reacts with: human (P08247), rat (P07825), mouse (Q62277), mammals.
Weaker signal: zebrafish, other vertebrates.
Other species not tested yet.
Specificity K.O. validated
Remarks

Widely used as marker for nerve terminals and neuroendocrine tumors. For unknown reason, neuronal synaptophysin is better recognised than neuroendocrine synaptophysin. If this is a problem, the polyclonal rabbit antibody, cat. no. 101 002 or 101 203 are recommended.
Data sheet 101_011c3.pdf

References for Synaptophysin1 - 101 011C3

Selective endocytosis of Ca2+-permeable AMPARs by the Alzheimer's disease risk factor CALM bidirectionally controls synaptic plasticity.
Azarnia Tehran D, Kochlamazashvili G, Pampaloni NP, Sposini S, Shergill JK, Lehmann M, Pashkova N, Schmidt C, Löwe D, Napieczynska H, Heuser A, et al.
Science advances (2022) 821: eabl5032. 101 011C3 WB; tested species: mouse
Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment.
Smolek T, Madari A, Farbakova J, Kandrac O, Jadhav S, Cente M, Brezovakova V, Novak M, Zilka N
The Journal of comparative neurology (2016) 5244: 874-95. 101 011C3 WB
The activation of mGluR4 rescues parallel fiber synaptic transmission and LTP, motor learning and social behavior in a mouse model of Fragile X Syndrome.
Martín R, Suárez-Pinilla AS, García-Font N, Laguna-Luque ML, López-Ramos JC, Oset-Gasque MJ, Gruart A, Delgado-García JM, Torres M, Sánchez-Prieto J
Molecular autism (2023) 141: 14. 101 011C3 ICC; tested species: mouse
Transient oxytocin signaling primes the development and function of excitatory hippocampal neurons.
Ripamonti S, Ambrozkiewicz MC, Guzzi F, Gravati M, Biella G, Bormuth I, Hammer M, Tuffy LP, Sigler A, Kawabe H, Nishimori K, et al.
eLife (2017) 6: . 101 011C3 ICC; tested species: mouse
Synapse formation and function is modulated by the amyloid precursor protein.
Priller C, Bauer T, Mitteregger G, Krebs B, Kretzschmar HA, Herms J
The Journal of neuroscience : the official journal of the Society for Neuroscience (2006) 2627: 7212-21. 101 011C3 ICC
Conditioning- and reward-related dendritic and presynaptic plasticity of nucleus accumbens neurons in male and female sign-tracker rats.
Colom M, Kraev I, Stramek AK, Loza IB, Rostron CL, Heath CJ, Dommett EJ, Singer BF
The European journal of neuroscience (2024) 607: 5694-5717. 101 011C3 IHC; tested species: rat
Alterations in neuromuscular junction morphology with ageing and endurance training modulate neuromuscular transmission and myofibre composition.
Yamaguchi T, Kouzaki K, Sasaki K, Nakazato K
The Journal of physiology (2024) : . 101 011C3 IHC; tested species: mouse
Impaired excitatory drive to spinal GABAergic neurons of neuropathic mice.
Leitner J, Westerholz S, Heinke B, Forsthuber L, Wunderbaldinger G, Jäger T, Gruber-Schoffnegger D, Braun K, Sandkühler J
PloS one (2013) 88: e73370. 101 011C3 IHC; tested species: mouse
Cat. No.: 101 011C3
Amount: 50 µg
Price: $475.00
Selective endocytosis of Ca2+-permeable AMPARs by the Alzheimer's disease risk factor CALM bidirectionally controls synaptic plasticity.
Azarnia Tehran D, Kochlamazashvili G, Pampaloni NP, Sposini S, Shergill JK, Lehmann M, Pashkova N, Schmidt C, Löwe D, Napieczynska H, Heuser A, et al.
Science advances (2022) 821: eabl5032. 101 011C3 WB; tested species: mouse
Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment.
Smolek T, Madari A, Farbakova J, Kandrac O, Jadhav S, Cente M, Brezovakova V, Novak M, Zilka N
The Journal of comparative neurology (2016) 5244: 874-95. 101 011C3 WB
The activation of mGluR4 rescues parallel fiber synaptic transmission and LTP, motor learning and social behavior in a mouse model of Fragile X Syndrome.
Martín R, Suárez-Pinilla AS, García-Font N, Laguna-Luque ML, López-Ramos JC, Oset-Gasque MJ, Gruart A, Delgado-García JM, Torres M, Sánchez-Prieto J
Molecular autism (2023) 141: 14. 101 011C3 ICC; tested species: mouse
Transient oxytocin signaling primes the development and function of excitatory hippocampal neurons.
Ripamonti S, Ambrozkiewicz MC, Guzzi F, Gravati M, Biella G, Bormuth I, Hammer M, Tuffy LP, Sigler A, Kawabe H, Nishimori K, et al.
eLife (2017) 6: . 101 011C3 ICC; tested species: mouse
Synapse formation and function is modulated by the amyloid precursor protein.
Priller C, Bauer T, Mitteregger G, Krebs B, Kretzschmar HA, Herms J
The Journal of neuroscience : the official journal of the Society for Neuroscience (2006) 2627: 7212-21. 101 011C3 ICC
Conditioning- and reward-related dendritic and presynaptic plasticity of nucleus accumbens neurons in male and female sign-tracker rats.
Colom M, Kraev I, Stramek AK, Loza IB, Rostron CL, Heath CJ, Dommett EJ, Singer BF
The European journal of neuroscience (2024) 607: 5694-5717. 101 011C3 IHC; tested species: rat
Alterations in neuromuscular junction morphology with ageing and endurance training modulate neuromuscular transmission and myofibre composition.
Yamaguchi T, Kouzaki K, Sasaki K, Nakazato K
The Journal of physiology (2024) : . 101 011C3 IHC; tested species: mouse
Impaired excitatory drive to spinal GABAergic neurons of neuropathic mice.
Leitner J, Westerholz S, Heinke B, Forsthuber L, Wunderbaldinger G, Jäger T, Gruber-Schoffnegger D, Braun K, Sandkühler J
PloS one (2013) 88: e73370. 101 011C3 IHC; tested species: mouse
ICC
IHC
Background

Synaptophysin1, also referred to as p38-1, is a membrane glycoprotein of synaptic vesicles that is ubiquitously expressed in all neurons and in many endocrine cells. It is currently the most widely used marker for nerve terminals and probably the best marker for the pathologist in differentiating neuroendocrine tumors.
Synaptophysin1 has four transmembrane domains with both N- and C-terminus facing the cytoplasm. It binds to synaptobrevin1 and synaptobrevin2 in detergent extracts but its function has not been elucidated completely. It forms a complex with dynamin at high Ca2+ concentration suggesting an involvement in synaptic vesicle endocytosis. As typical for synaptic vesicle proteins, synaptophysin1 represents a small protein family with two additonal members, synaptoporin (synaptophysin2) and panthophysin. Like synaptophysin1, synaptoporin is widely expressed in neurons and colocalizes with synaptophysin1 on synaptic vesicles whereas panthophysin is expressed in all tissues.
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