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Synaptophysin 1 - 101 011C3

Major synaptic vesicle membrane protein; neuroendocrine tumor marker
Monoclonal mouse purified IgG
Cat. No.: 101 011C3
Amount: 50 µg
Price: $465.00
Cat. No. 101 011C3 50 µg purified IgG, lyophilized, fluorescence-labeled with Oyster® 550

Fluorescence labeled antibodies conjugated to Oyster dyes from Luminartis GmbH (formerly Denovo Biolabels) allow the usage of established CyDye fluorescence detection systems. Oyster-488 has similar exitation and emission properties as Cy2, Oyster-550 as Cy3, and Oyster-650 is comparable to Cy5.
For our custom antibody labeling service Oyster-500 is also available.
Oyster dyes do not form dimers and therefore exhibit less internal quenching. This allows a higher labeling degree and consequently brighter conjugates.

Oyster dyes, Oyster-488, Oyster-500, Oyster-550, and Oyster-650 are trademarks of Luminartis GmbH, Muenster/Germany.

CyDyes, Cy2, Cy3, and Cy5 are trademarks of GE-Healthcare Ltd.

. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.The mounting agent Aquatex®(Merck Chemicals) is not compatible with Oyster dyes!
Applications WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P/FFPE: not tested yet
Label Oyster 550
Clone 7.2
Subtype IgG1 (λ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 307 from rat Synaptophysin1 (UniProt Id: P07825)
Epitop Epitop: AA 219 to 307 from rat Synaptophysin1 (UniProt Id: P07825)
Reactivity Reacts with: human (P08247), rat (P07825), mouse (Q62277), mammals.
Weaker signal: zebrafish, other vertebrates.
Other species not tested yet.
Specificity Specific for synaptophysin 1, no cross-reactivity to other synaptophysins. K.O.
Remarks

Widely used as marker for nerve terminals and neuroendocrine tumors. For unknown reason, neuronal synaptophysin is better recognised than neuroendocrine synaptophysin. If this is a problem, the polyclonal rabbit antibody, cat. no. 101 002 or 101 203 are recommended.

Data sheet 101_011c3.pdf

References for Synaptophysin 1 - 101 011C3

Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment.
Smolek T, Madari A, Farbakova J, Kandrac O, Jadhav S, Cente M, Brezovakova V, Novak M, Zilka N
The Journal of comparative neurology (2016) 5244: 874-95. 101 011C3 WB
Transient oxytocin signaling primes the development and function of excitatory hippocampal neurons.
Ripamonti S, Ambrozkiewicz MC, Guzzi F, Gravati M, Biella G, Bormuth I, Hammer M, Tuffy LP, Sigler A, Kawabe H, Nishimori K, et al.
eLife (2017) 6: . 101 011C3 ICC; tested species: mouse
Synapse formation and function is modulated by the amyloid precursor protein.
Priller C, Bauer T, Mitteregger G, Krebs B, Kretzschmar HA, Herms J
The Journal of neuroscience : the official journal of the Society for Neuroscience (2006) 2627: 7212-21. 101 011C3 ICC
Impaired excitatory drive to spinal GABAergic neurons of neuropathic mice.
Leitner J, Westerholz S, Heinke B, Forsthuber L, Wunderbaldinger G, Jäger T, Gruber-Schoffnegger D, Braun K, Sandkühler J
PloS one (2013) 88: e73370. 101 011C3 IHC; tested species: mouse
Cat. No.: 101 011C3
Quantity: 50 µg
Price: $465.00
Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment.
Smolek T, Madari A, Farbakova J, Kandrac O, Jadhav S, Cente M, Brezovakova V, Novak M, Zilka N
The Journal of comparative neurology (2016) 5244: 874-95. 101 011C3 WB
Transient oxytocin signaling primes the development and function of excitatory hippocampal neurons.
Ripamonti S, Ambrozkiewicz MC, Guzzi F, Gravati M, Biella G, Bormuth I, Hammer M, Tuffy LP, Sigler A, Kawabe H, Nishimori K, et al.
eLife (2017) 6: . 101 011C3 ICC; tested species: mouse
Synapse formation and function is modulated by the amyloid precursor protein.
Priller C, Bauer T, Mitteregger G, Krebs B, Kretzschmar HA, Herms J
The Journal of neuroscience : the official journal of the Society for Neuroscience (2006) 2627: 7212-21. 101 011C3 ICC
Impaired excitatory drive to spinal GABAergic neurons of neuropathic mice.
Leitner J, Westerholz S, Heinke B, Forsthuber L, Wunderbaldinger G, Jäger T, Gruber-Schoffnegger D, Braun K, Sandkühler J
PloS one (2013) 88: e73370. 101 011C3 IHC; tested species: mouse
Background

Synaptophysin 1, also referred to as p38-1, is a membrane glycoprotein of synaptic vesicles that is ubiquitously expressed in all neurons and in many endocrine cells. It is currently the most widely used marker for nerve terminals and probably the best marker for the pathologist in differentiating neuroendocrine tumors.
Synaptophysin 1 has four transmembrane domains with both N- and C-terminus facing the cytoplasm. It binds to synaptobrevin 1 and synaptobrevin 2 in detergent extracts but its function has not been elucidated completely. It forms a complex with dynamin at high Ca2+ concentration suggesting an involvement in synaptic vesicle endocytosis. As typical for synaptic vesicle proteins, synaptophysin 1 represents a small protein family with two additonal members, synaptoporin (synaptophysin 2) and panthophysin. Like synaptophysin 1, synaptoporin is widely expressed in neurons and colocalizes with synaptophysin 1 on synaptic vesicles whereas panthophysin is expressed in all tissues.