Synaptic Systems - Synaptophysin1

Cat. No.: 101 011BT

Amount: 50 µg

Price: $475.00

WB

ICC

IHC

IHC-P

Background

Synaptophysin1, also referred to as p38-1, is a membrane glycoprotein of synaptic vesicles that is ubiquitously expressed in all neurons and in many endocrine cells. It is currently the most widely used marker for nerve terminals and probably the best marker for the pathologist in differentiating neuroendocrine tumors.
Synaptophysin1 has four transmembrane domains with both N- and C-terminus facing the cytoplasm. It binds to synaptobrevin1 and synaptobrevin2 in detergent extracts but its function has not been elucidated completely. It forms a complex with dynamin at high Ca²⁺ concentration suggesting an involvement in synaptic vesicle endocytosis. As typical for synaptic vesicle proteins, synaptophysin1 represents a small protein family with two additonal members, synaptoporin (synaptophysin2) and panthophysin. Like synaptophysin1, synaptoporin is widely expressed in neurons and colocalizes with synaptophysin1 on synaptic vesicles whereas panthophysin is expressed in all tissues.

1 ) Janz R et al., Neuron (1999)
2 ) McMahon HT et al., Proc. Natl. Acad. Sci. U.S.A. (1996)
3 ) Südhof TC et al., Nature (1995)
4 ) Buckley KM et al., J. Cell Biol. (1987)
5 ) Leube RE et al., EMBO J. (1987)
6 ) Südhof TC et al., Science (1987)

Protocols

WB

IP

Standard protocol

ICC

IHC

IHC-P

Other products for this target

Cat. No. 101 011BT	50 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 10000 (AP staining) gallery IP: yes ICC: 1 : 100 up to 1 : 1000 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 100 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Clone	7.2
Subtype	IgG1 (λ light chain)
Immunogen	Full-length recombinant rat Synaptophysin (UniProt Id: P07825)
Epitop	AA 219 to 307 from rat Synaptophysin1 (UniProt Id: P07825)
Reactivity	Reacts with: human (P08247), rat (P07825), mouse (Q62277), mammals. Weaker signal: zebrafish, other vertebrates. Other species not tested yet.
Specificity	K.O. validated
Remarks	Widely used as marker for nerve terminals and neuroendocrine tumors. For unknown reason, neuronal synaptophysin is better recognised than neuroendocrine synaptophysin. If this is a problem, the polyclonal rabbit antibody, cat. no. 101 002 or 101 203 are recommended.
Data sheet	101_011bt.pdf

Synaptophysin1 antibody - 101 011BT K.O.