|Cat. No. 101 011BT||50 µg purified IgG, lyophilized, biotin-labeled. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 10000 (AP staining) gallery
ICC: 1 : 100 up to 1 : 1000 gallery
IHC: 1 : 200 up to 1 : 500 gallery
IHC-P/FFPE: 1 : 100 gallery
|Subtype||IgG1 (λ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 307 from rat Synaptophysin1 (UniProt Id: P07825)|
Epitop: AA 219 to 307 from rat Synaptophysin1 (UniProt Id: P07825)
Reacts with: human (P08247), rat (P07825), mouse (Q62277), mammals.
Weaker signal: zebrafish, other vertebrates.
Other species not tested yet.
|Specificity||Specific for synaptophysin 1, no cross-reactivity to other synaptophysins. K.O.|
Widely used as marker for nerve terminals and neuroendocrine tumors. For unknown reason, neuronal synaptophysin is better recognised than neuroendocrine synaptophysin. If this is a problem, the polyclonal rabbit antibody, cat. no. 101 002 or 101 203 are recommended.
Synaptophysin 1, also referred to as p38-1, is a membrane glycoprotein of synaptic vesicles that is ubiquitously expressed in all neurons and in many endocrine cells. It is currently the most widely used marker for nerve terminals and probably the best marker for the pathologist in differentiating neuroendocrine tumors.
Synaptophysin 1 has four transmembrane domains with both N- and C-terminus facing the cytoplasm. It binds to synaptobrevin 1 and synaptobrevin 2 in detergent extracts but its function has not been elucidated completely. It forms a complex with dynamin at high Ca2+ concentration suggesting an involvement in synaptic vesicle endocytosis. As typical for synaptic vesicle proteins, synaptophysin 1 represents a small protein family with two additonal members, synaptoporin (synaptophysin 2) and panthophysin. Like synaptophysin 1, synaptoporin is widely expressed in neurons and colocalizes with synaptophysin 1 on synaptic vesicles whereas panthophysin is expressed in all tissues.