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Synaptophysin 1 - 101 008

Major synaptic vesicle membrane protein; neuroendocrine tumor marker
Monoclonal Recombinant rabbit purified IgG
Cat. No.: 101 008
Amount: 50 µg
Price: 415.00 €
Cat. No. 101 008 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 500 gallery  
Clone Rb7.2
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 307 from rat Synaptophysin1 (UniProt Id: P07825)
Epitop Epitop: AA 219 to 307 from rat Synaptophysin1 (UniProt Id: P07825)
Reactivity Reacts with: human (P08247), rat (P07825), mouse (Q62277), mammals.
Weaker signal: zebrafish, other vertebrates.
Other species not tested yet.
Specificity Specific for synaptophysin 1, no cross-reactivity to other synaptophysins. K.O.
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 7.2. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Synaptophysin 1 is widely used as marker for nerve terminals and neuroendocrine tumors. For still unknown reason, neuronal synaptophysin is better recognized than neuroendocrine synaptophysin. If this is a problem, the polyclonal rabbit antibody, cat. no. 101 002, is recommended.

Data sheet 101_008.pdf
Cat. No.: 101 008
Quantity: 50 µg
Price: 415.00 €
Background

Synaptophysin 1, also referred to as p38-1, is a membrane glycoprotein of synaptic vesicles that is ubiquitously expressed in all neurons and in many endocrine cells. It is currently the most widely used marker for nerve terminals and probably the best marker for the pathologist in differentiating neuroendocrine tumors.
Synaptophysin 1 has four transmembrane domains with both N- and C-terminus facing the cytoplasm. It binds to synaptobrevin 1 and synaptobrevin 2 in detergent extracts but its function has not been elucidated completely. It forms a complex with dynamin at high Ca2+ concentration suggesting an involvement in synaptic vesicle endocytosis. As typical for synaptic vesicle proteins, synaptophysin 1 represents a small protein family with two additonal members, synaptoporin (synaptophysin 2) and panthophysin. Like synaptophysin 1, synaptoporin is widely expressed in neurons and colocalizes with synaptophysin 1 on synaptic vesicles whereas panthophysin is expressed in all tissues.