Cat. No.: 131 011BT
Amount: 100 µg
Price:
$475.00
Cat. No. 131 011BT |
100 µg purified IgG, lyophilized, labeled with Biotin.
Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: yes Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 200 up to 1 : 1000 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 100 up to 1 : 1000 gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet |
Label | biotin |
Clone | 117G4 |
Subtype | IgG3 (κ light chain) |
Immunogen | Synthetic peptide corresponding to residues near the amino terminus of rat VGAT (UniProt Id: O35458) |
Reactivity |
Reacts with: human (Q9H598), rat (O35458), mouse (O35633), Guinea pig, monkey. Other species not tested yet. |
Specificity | K.O. validated |
Matching control protein/peptide | 131-0P |
Remarks |
WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. |
Data sheet | 131_011bt.pdf |