VAP-A antibody - 249 002

VAP-A is a SNARE binding protein

Rabbit polyclonal antiserum

Cat. No.: 249 002

Amount: 200 µl

Price: $355.00

Cat. No. 249 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 223 from human VAP-A (UniProt Id: Q9P0L0)
Reactivity	Reacts with: human (Q9P0L0), rat (Q9Z270), mouse (Q9WV55). Other species not tested yet.
Matching control protein/peptide	249-0P
Data sheet	249_002.pdf

References for VAP-A - 249 002

Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cell.
Cepeda AP, Ninov M, Neef J, Parfentev I, Kusch K, Reisinger E, Jahn R, Moser T, Urlaub H
Molecular & cellular proteomics : MCP (2023) : 100704. 249 002 IHC; tested species: mouse

Cat. No.: 249 002

Amount: 200 µl

Price: $355.00

Background

The VAMP-associated protein A VAP-A, also referred to as VAP-33, is a ubiquitously expressed type IV membrane protein. It is composed of an N-terminal domain with an immunoglobulin-like b-sheet, a central coiled-coiled domain and a C-terminal transmembrane domain. The protein, which may be involved in vesicle trafficking, is located to the endoplasmic reticulum (ER) and ER/Golgi intermediate compartment (ERGIC). VAP-A has been shown to bind to dfifferent v- and t-SNARE proteins.
Two other isoforms, VAP-B and VAP-C, have been described in the literature so far.

1 ) Tuuf J et al., Biochem. Biophys. Res. Commun. (2009)
2 ) Ettayebi K et al., J. Virol. (2003)
3 ) Wyles JP et al., J. Biol. Chem. (2002)
4 ) Weir ML et al., Biochem. Biophys. Res. Commun. (2001)

Protocols

OTHER

Preabsorbtion

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