VAP-A protein - 249-0P

VAP-A is a SNARE binding protein

control protein

Cat. No.: 249-0P

Amount: 100 µg

Price: $105.00

Cat. No. 249-0P	100 µg protein, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
Applications
Immunogen	Recombinant protein corresponding to AA 1 to 223 from human VAP-A (UniProt Id: Q9P0L0)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	249 002, 249 003
Remarks	This control protein consists of the recombinant protein (aa 1-223 of human VAP-A) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	249-0p.pdf

Cat. No.: 249-0P

Amount: 100 µg

Price: $105.00

Background

The VAMP-associated protein A VAP-A, also referred to as VAP-33, is a ubiquitously expressed type IV membrane protein. It is composed of an N-terminal domain with an immunoglobulin-like b-sheet, a central coiled-coiled domain and a C-terminal transmembrane domain. The protein, which may be involved in vesicle trafficking, is located to the endoplasmic reticulum (ER) and ER/Golgi intermediate compartment (ERGIC). VAP-A has been shown to bind to dfifferent v- and t-SNARE proteins.
Two other isoforms, VAP-B and VAP-C, have been described in the literature so far.

1 ) Tuuf J et al., Biochem. Biophys. Res. Commun. (2009)
2 ) Ettayebi K et al., J. Virol. (2003)
3 ) Wyles JP et al., J. Biol. Chem. (2002)
4 ) Weir ML et al., Biochem. Biophys. Res. Commun. (2001)

Protocols

OTHER

Preadsorbtion

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