Cat. No.: 302 203
Amount: 100 µg
Price:
$375.00
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| Cat. No. 302 203 |
100 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Synthetic peptide corresponding to AA 443 to 449 from rat α-tubulin (UniProt Id: P68370) (UniProt Id: P68370-1) |
| Reactivity |
Reacts with: human, rat (P68370-1), mouse, mammals, chicken. Other species not tested yet. |
| Specificity | Recognizes glu- and tyr-α-tubulin and Δ2-tubulin with a preference for glu- and Δ2-tubulin. |
| Remarks |
ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 302 211 as capture antibodies. |
| Data sheet | 302_203.pdf |
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Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter.
Assembled microtubules can be detyrosinated by a carboxypeptidaseS called vasohibins / SVBPs. Detyrosinated α-tubulin is referred to as Glu-α-tubulin and occurs for exemple in neurons. This reaction can be reverted by Tubulin tyrosine ligase (TTL) that ads a C-terminal tyrosin to Glu α-tubulin.
Another post-translational modification of α-tubulin is C-terminal polyglutamylation which is also characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by TTL and is one of the dominant α-tubulin isoforms in neurons.