Synaptic Systems - alpha/beta Synuclein

Cat. No.: 128 003

Amount: 50 µg

Price: $455.00

Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues.
Klevanski M, Herrmannsdoerfer F, Sass S, Venkataramani V, Heilemann M, Kuner T
Nature communications (2020) 111: 1552. 128 003 IHC; tested species: rat

WB

ICC

IHC

IHC-P

Background

Synuclein proteins are produced by three genes. They share structural resemblance to apolipoproteins, but are abundant in the neuronal cytosol and present in enriched amounts at presynaptic terminals.
Synucleins have been specifically implicated in three diseases: Alzheimer's (AD), Parkinson's (PD) and breast cancer. In AD, a peptide derived from α-synuclein forms an intrinsic component of plaque amyloid. In PD, an α-synuclein allele is genetically linked to several independent familial cases, and the protein appears to accumulate in Lewy bodies. In breast cancer, increased expression of γ-synuclein correlates with disease progression.
In songbirds, α-synuclein expression is correlated with plasticity in the developing song control system. Although the normal function of synucleins is unknown, a role in synaptic plasticity seems likely.

1 ) Polymeropoulos MH et al., Ann. N. Y. Acad. Sci. (2000)
2 ) Stefanova N et al., J. Neural Transm. Suppl. (2000)
3 ) Clayton DF et al., Trends Neurosci. (1998)
4 ) Goedert M et al., Curr. Opin. Neurobiol. (1998)
5 ) Hardy J et al., Science (1998)
6 ) Lavedan C et al., Genome Res. (1998)
7 ) Lozano AM et al., Curr. Opin. Neurobiol. (1998)

Protocols

WB

IP

Standard protocol

ICC

IHC

IHC-P

ELISA

Standard protocol

Other products for this target

Cat. No. 128 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 200 gallery ELISA: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen	Synthetic peptide corresponding to AA 2 to 25 from human α-Synuclein (UniProt Id: P37840)
Reactivity	Reacts with: rat (P37377, Q63754), mouse (O55042, Q91ZZ3), zebrafish, human (P37840, Q16143). Other species not tested yet.
Specificity	Recognizes α synuclein and β synuclein.
Remarks	ELISA: This antibody is suitable as detector antibody for sandwich-ELISA with cat. no. 128 011 or 128 211 as capture antibodies.
Data sheet	128_003.pdf

alpha/beta Synuclein antibody - 128 003

References for alpha/beta Synuclein - 128 003