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Abeta 38/40/42/43 - 218 211

A major component of neuritic plaques in Alzheimer's disease
Monoclonal mouse purified IgG
Cat. No.: 218 211
Amount: 100 µg
Price: 415.00 €
Cat. No. 218 211 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P/FFPE: 1 : 1000 up to 1 : 2000 gallery  
ELISA: yes (see remarks)
Clone NT244
Subtype IgG2b (κ light chain)
Immunogen Synthetic peptide corresponding to AA 1 to 16 from human Abeta (UniProt Id: P05067)
Epitop Epitop: AA 4 to 8 from human Abeta (UniProt Id: P05067)
Reactivity Reacts with: human (P05067), mouse (P12023).
Other species not tested yet.
Specificity Specific for Abeta 38, 40, 42, 43.
Remarks

WB: Boil membrane after blotting for 3min.

IHC: Antigen retrieval with formic acid is required.

ELISA: Suitable as detector antibodies with cat.no. 218 203, 218 403 and 218 703 as capture antibodies.

Data sheet 218_211.pdf

References for Abeta 38/40/42/43 - 218 211

Oxidative Stress during the Progression of β-Amyloid Pathology in the Neocortex of the Tg2576 Mouse Model of Alzheimer's Disease.
Porcellotti S, Fanelli F, Fracassi A, Sepe S, Cecconi F, Bernardi C, Cimini A, Cerù MP, Moreno S
Oxidative medicine and cellular longevity (2015) 2015: 967203. 218 211 IHC
Aβ38 in the brains of patients with sporadic and familial Alzheimer's disease and transgenic mouse models.
Reinert J, Martens H, Huettenrauch M, Kolbow T, Lannfelt L, Ingelsson M, Paetau A, Verkkoniemi-Ahola A, Bayer TA, Wirths O
Journal of Alzheimer's disease : JAD (2014) 394: 871-81. 218 211 IHC; tested species: human
Oligomeric pyroglutamate amyloid-β is present in microglia and a subfraction of vessels in patients with Alzheimer's disease: implications for immunotherapy.
Wirths O, Hillmann A, Pradier L, Härtig W, Bayer TA
Journal of Alzheimer's disease : JAD (2013) 354: 741-9. 218 211 IHC; tested species: human
Antibody 9D5 recognizes oligomeric pyroglutamate amyloid-β in a fraction of amyloid-β deposits in Alzheimer's disease without cross-reactivity with other protein aggregates.
Venkataramani V, Wirths O, Budka H, Härtig W, Kovacs GG, Bayer TA
Journal of Alzheimer's disease : JAD (2012) 292: 361-71. 218 211 IHC; tested species: human
Cat. No.: 218 211
Quantity: 100 µg
Price: 415.00 €
Oxidative Stress during the Progression of β-Amyloid Pathology in the Neocortex of the Tg2576 Mouse Model of Alzheimer's Disease.
Porcellotti S, Fanelli F, Fracassi A, Sepe S, Cecconi F, Bernardi C, Cimini A, Cerù MP, Moreno S
Oxidative medicine and cellular longevity (2015) 2015: 967203. 218 211 IHC
Aβ38 in the brains of patients with sporadic and familial Alzheimer's disease and transgenic mouse models.
Reinert J, Martens H, Huettenrauch M, Kolbow T, Lannfelt L, Ingelsson M, Paetau A, Verkkoniemi-Ahola A, Bayer TA, Wirths O
Journal of Alzheimer's disease : JAD (2014) 394: 871-81. 218 211 IHC; tested species: human
Oligomeric pyroglutamate amyloid-β is present in microglia and a subfraction of vessels in patients with Alzheimer's disease: implications for immunotherapy.
Wirths O, Hillmann A, Pradier L, Härtig W, Bayer TA
Journal of Alzheimer's disease : JAD (2013) 354: 741-9. 218 211 IHC; tested species: human
Antibody 9D5 recognizes oligomeric pyroglutamate amyloid-β in a fraction of amyloid-β deposits in Alzheimer's disease without cross-reactivity with other protein aggregates.
Venkataramani V, Wirths O, Budka H, Härtig W, Kovacs GG, Bayer TA
Journal of Alzheimer's disease : JAD (2012) 292: 361-71. 218 211 IHC; tested species: human
Background

Amyloid deposits, also called plaques, of Alzheimer's patients consist of several protein components like the amyloid beta-peptides (Abeta, Aβ) 1-40/42/43 and additional C- and N-terminally modified fragments of Abeta as for instance Abeta pE3 and Abeta pE11.
An additional Abeta variant, Abeta 38, is more soluble compared to other Abeta species and is not found in plaques of sporadic Alzheimer´s cases. However, it is detected in the blood-vessel walls of a subset of patients with severe cerebral amyloid angiopathy. It especially accumulates in brains of patients carrying mutations in the Abeta coding region.
Cleavage of amyloid precursor protein APP by β- and γ- secretases results in the generation of the Aβ (βA4)peptide, whereas α-secretase cleaves within the Aβ sequence and prevents the formation of Abeta from APP.