Cat. No.: 399 003
Amount: 50 µg
Price:
$375.00
Cat. No. 399 003 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 up to 1 : 1000 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 up to 1 : 1000 gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 500 gallery In antibody-based DNA-PAINT (Point Accumulation in Nanoscale Topography), a short oligomeric docking strand is coupled to a specific antibody. The transient association of the fluorophore to the antibody is mediated by the pairing of a short fluorescently labeled complementary imager DNA strand. DNA-PAINT allows super-resolution imaging and the imaging of a huge number of antibodies on the same biological sample in a single multiplex experiment. For additional experimental details refer to the Remarks section.', $event)" style="cursor: help;">DNA-PAINT: yes (see remarks) |
Immunogen | Synthetic peptide corresponding to AA 290 to 302 from rat Spinophilin (UniProt Id: O35274) |
Reactivity |
Reacts with: rat (O35274), mouse (Q6R891). Other species not tested yet. |
Specificity | Specific for Spinophilin isoform1. Does not detect isoform 2. K.O. validated |
Matching control protein/peptide | 399-0P |
Remarks |
DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614). |
Data sheet | 399_003.pdf |