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Shank1 antibody - 162 013 K.O.

Shanks are multidomain proteins of the postsynaptic density
Rabbit polyclonal purified antibody
Cat. No.: 162 013
Amount: 50 µg
Price: $455.00
Cat. No. 162 013 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
IHC-Fr: 1 : 500 (see remarks) gallery  
Immunogen Recombinant protein corresponding to AA 397 to 619 from rat Shank1 (UniProt Id: Q9WV48)
Reactivity Reacts with: rat (Q9WV48), mouse (D3YZU1).
Other species not tested yet.
Specificity Specific for shank 1 with weak cross reactivity to shank 2 and 3 due to high sequence homology. K.O. validated PubMed: 25816842
Remarks

ICC: Methanol fixation is recommended.
IHC-Fr: Fixation with acetone, methanol or PFA/formaldehyde is recommended. Signal intensities as follows: acetone > methanol > PFA.

Data sheet 162_013.pdf

References for Shank1 - 162 013

Shank1 regulates excitatory synaptic transmission in mouse hippocampal parvalbumin-expressing inhibitory interneurons.
Mao W, Watanabe T, Cho S, Frost JL, Truong T, Zhao X, Futai K
The European journal of neuroscience (2015) 418: 1025-35. 162 013 ICC, IHC; KO verified
Selective targeting of unipolar brush cell subtypes by cerebellar mossy fibers.
Balmer TS, Trussell LO
eLife (2019) 8: . 162 013 IHC; tested species: mouse
Shank1 regulates excitatory synaptic transmission in mouse hippocampal parvalbumin-expressing inhibitory interneurons.
Mao W, Watanabe T, Cho S, Frost JL, Truong T, Zhao X, Futai K
The European journal of neuroscience (2015) 418: 1025-35. 162 013 ICC, IHC; KO verified
Cat. No.: 162 013
Amount: 50 µg
Price: $455.00
Shank1 regulates excitatory synaptic transmission in mouse hippocampal parvalbumin-expressing inhibitory interneurons.
Mao W, Watanabe T, Cho S, Frost JL, Truong T, Zhao X, Futai K
The European journal of neuroscience (2015) 418: 1025-35. 162 013 ICC, IHC; KO verified
Selective targeting of unipolar brush cell subtypes by cerebellar mossy fibers.
Balmer TS, Trussell LO
eLife (2019) 8: . 162 013 IHC; tested species: mouse
Shank1 regulates excitatory synaptic transmission in mouse hippocampal parvalbumin-expressing inhibitory interneurons.
Mao W, Watanabe T, Cho S, Frost JL, Truong T, Zhao X, Futai K
The European journal of neuroscience (2015) 418: 1025-35. 162 013 ICC, IHC; KO verified