Cat. No.: 186 003
Amount: 50 µg
Price:
$380.00
Cat. No. 186 003 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 100 up to 1 : 500 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: not tested yet Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.', $event)" style="cursor: help;">EM: not tested yet |
Immunogen | Recombinant protein corresponding to rat Sec22b lacking the membrane anchor. (UniProt Id: Q4KM74) |
Reactivity |
Reacts with: human (O75396), rat (Q4KM74), mouse (O08547), zebrafish. Other species not tested yet. |
Specificity | K.O. validated PubMed: 36595686 |
Remarks |
This antibody has been purified with a synthetic peptide corresponding to AA 87-100 from rat Sec22b. |
Data sheet | 186_003.pdf |
Distinct sets of SNARE proteins mediate membrane-membrane fusion events between different intracellular compartments. Sec 22b (ERS 24, rsec22b), a Sly2 homolog, is a R-SNARE and locates to the endoplasmatic reticulum (ER) and the Golgi apparatus. Together with syntaxin 5, membrin and Bet 1 it participates in traffic between the Golgi and the ER.