Synaptic Systems

Cat. No.: 186 003

Amount: 50 µg

Price: $380.00

Cat. No. 186 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: 1 : 100 up to 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet EM: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen	Recombinant protein corresponding to rat Sec22b lacking the membrane anchor. (UniProt Id: Q4KM74)
Reactivity	Reacts with: human (O75396), rat (Q4KM74), mouse (O08547), zebrafish. Other species not tested yet.
Specificity	K.O. validated PubMed: 36595686
Remarks	This antibody has been purified with a synthetic peptide corresponding to AA 87-100 from rat Sec22b.
Data sheet	186_003.pdf

References for Sec22b - 186 003

Sec22b regulates phagosome maturation by promoting ORP8-mediated lipid exchange at endoplasmic reticulum-phagosome contact sites.
Criado Santos N, Bouvet S, Cruz Cobo M, Mandavit M, Bermont F, Castelbou C, Mansour F, Azam M, Giordano F, Nunes-Hasler P
Communications biology (2023) 61: 1008. 186 003 WB, ICC; KD verified; tested species: mouse

Sec22b is a critical and nonredundant regulator of plasma cell maintenance.
Bonaud A, Gargowitsch L, Gilbert SM, Rajan E, Canales-Herrerias P, Stockholm D, Rahman NF, Collins MO, Taskiran H, Hill DL, Alloatti A, et al.
Proceedings of the National Academy of Sciences of the United States of America (2023) 1202: e2213056120. 186 003 WB; KO verified; tested species: mouse

Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.
Xie YX, Naseri NN, Fels J, Kharel P, Na Y, Lane D, Burré J, Sharma M
Nature communications (2022) 131: 4918. 186 003 WB; tested species: mouse

Sec22b Regulates Inflammatory Responses by Controlling the Nuclear Translocation of NF-κB and the Secretion of Inflammatory Mediators.
Arango Duque G, Dion R, Matte C, Fabié A, Descoteaux J, Stäger S, Descoteaux A
Journal of immunology (Baltimore, Md. : 1950) (2021) 2079: 2297-2309. 186 003 WB, ICC, EM; KD verified; tested species: mouse

TAP dysfunction in dendritic cells enables noncanonical cross-presentation for T cell priming.
Barbet G, Nair-Gupta P, Schotsaert M, Yeung ST, Moretti J, Seyffer F, Metreveli G, Gardner T, Choi A, Tortorella D, Tampé R, et al.
Nature immunology (2021) 224: 497-509. 186 003 WB; KD verified; tested species: mouse

Organelle tethering, pore formation and SNARE compensation in the late endocytic pathway.
Davis LJ, Bright NA, Edgar JR, Parkinson MDJ, Wartosch L, Mantell J, Peden AA, Luzio JP
Journal of cell science (2021) 13410: . 186 003 WB; tested species: human

Activity of the SNARE Protein SNAP29 at the Endoplasmic Reticulum and Golgi Apparatus.
Morelli E, Speranza EA, Pellegrino E, Beznoussenko GV, Carminati F, Garré M, Mironov AA, Onorati M, Vaccari T
Frontiers in cell and developmental biology (2021) 9: 637565. 186 003 WB, IP, ICC; KD verified; tested species: human

Sec22b determines Weibel-Palade body length by controlling anterograde ER-Golgi transport.
Karampini E, Bürgisser PE, Olins J, Mulder AA, Jost CR, Geerts D, Voorberg J, Bierings R
Haematologica (2020) : . 186 003 WB, ICC; KD verified; tested species: human

A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science (2019) 13213: . 186 003 WB; tested species: human

The host cell secretory pathway mediates the export of Leishmania virulence factors out of the parasitophorous vacuole.
Arango Duque G, Jardim A, Gagnon É, Fukuda M, Descoteaux A
PLoS pathogens (2019) 157: e1007982. 186 003 WB, ICC, EM; KD verified; tested species: mouse

Autophagy induction via STING trafficking is a primordial function of the cGAS pathway.
Gui X, Yang H, Li T, Tan X, Shi P, Li M, Du F, Chen ZJ
Nature (2019) : . 186 003 WB; tested species: human

Identification of a Botulinum Neurotoxin-like Toxin in a Commensal Strain of Enterococcus faecium.
Zhang S, Lebreton F, Mansfield MJ, Miyashita SI, Zhang J, Schwartzman JA, Tao L, Masuyer G, Martínez-Carranza M, Stenmark P, Gilmore MS, et al.
Cell host & microbe (2018) 232: 169-176.e6. 186 003 WB; tested species: mouse

Identification and characterization of a novel botulinum neurotoxin.
Zhang S, Masuyer G, Zhang J, Shen Y, Lundin D, Henriksson L, Miyashita SI, Martínez-Carranza M, Dong M, Stenmark P
Nature communications (2017) 8: 14130. 186 003 WB; tested species: mouse

MHC I presentation of Toxoplasma gondii immunodominant antigen does not require Sec22b and is regulated by antigen orientation at the vacuole membrane.
Buaillon C, Guerrero NA, Cebrian I, Blanié S, Lopez J, Bassot E, Vasseur V, Santi-Rocca J, Blanchard N
European journal of immunology (2017) 477: 1160-1170. 186 003 WB; KD verified; tested species: mouse

Highly potent intracellular membrane-associated Aβ seeds.
Marzesco AM, Flötenmeyer M, Bühler A, Obermüller U, Staufenbiel M, Jucker M, Baumann F
Scientific reports (2016) 6: 28125. 186 003 WB

Sec22b regulates phagosomal maturation and antigen crosspresentation by dendritic cells.
Cebrian I, Visentin G, Blanchard N, Jouve M, Bobard A, Moita C, Enninga J, Moita LF, Amigorena S, Savina A
Cell (2011) 1476: 1355-68. 186 003 WB, ICC; tested species: mouse

Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 186 003 WB; tested species: rat

Regulation of Cx36 trafficking through the early secretory pathway by COPII cargo receptors and Grasp55.
Tetenborg S, Ariakia F, Martinez-Soler E, Shihabeddin E, Lazart IC, Miller AC, O'Brien J
Cellular and molecular life sciences : CMLS (2024) 811: 431. 186 003 ICC; tested species: human

The GTPase Rab39a promotes phagosome maturation into MHC-I antigen-presenting compartments.
Cruz FM, Colbert JD, Rock KL
The EMBO journal (2019) : e102020. 186 003 ICC; tested species: mouse

Cat. No.: 186 003

Amount: 50 µg

Price: $380.00

Autophagy induction via STING trafficking is a primordial function of the cGAS pathway.
Gui X, Yang H, Li T, Tan X, Shi P, Li M, Du F, Chen ZJ
Nature (2019) : . 186 003 WB; tested species: human

Highly potent intracellular membrane-associated Aβ seeds.
Marzesco AM, Flötenmeyer M, Bühler A, Obermüller U, Staufenbiel M, Jucker M, Baumann F
Scientific reports (2016) 6: 28125. 186 003 WB

The GTPase Rab39a promotes phagosome maturation into MHC-I antigen-presenting compartments.
Cruz FM, Colbert JD, Rock KL
The EMBO journal (2019) : e102020. 186 003 ICC; tested species: mouse

ICC

Background

Distinct sets of SNARE proteins mediate membrane-membrane fusion events between different intracellular compartments. Sec 22b (ERS 24, rsec22b), a Sly2 homolog, is a R-SNARE and locates to the endoplasmatic reticulum (ER) and the Golgi apparatus. Together with syntaxin 5, membrin and Bet 1 it participates in traffic between the Golgi and the ER.

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8 ) Newman AP et al., Mol. Cell. Biol. (1990)

Protocols

ICC

Sec22b antibody - 186 003 K.O.

References for Sec22b - 186 003