Synaptic Systems

Cat. No.: 121 003

Amount: 50 µg

Price: $455.00

Cat. No. 121 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 2 to 15 from rat SCAMP1 (UniProt Id: P56603)
Reactivity	Reacts with: human (O15126), rat (P56603), mouse (Q8K021), hamster. Other species not tested yet.
Matching control protein/peptide	121-0P
Data sheet	121_003.pdf

References for SCAMP1 - 121 003

Serotonin Transporter Associated Protein Complexes Are Enriched in Synaptic Vesicle Proteins and Proteins Involved in Energy Metabolism and Ion Homeostasis.
Haase J, Grudzinska-Goebel J, Müller HK, Münster-Wandowski A, Chow E, Wynne K, Farsi Z, Zander JF, Ahnert-Hilger G
ACS chemical neuroscience (2017) 85: 1101-1116. 121 003 WB; tested species: mouse,rat

Cat. No.: 121 003

Amount: 50 µg

Price: $455.00

ICC

IHC

IHC-P

Background

SCAMPs (secretory carrier membrane proteins) are general markers of membranes that function in cell surface recycling such as secretory vesicles, pancreatic granules, etc. They have four conserved transmembrane regions (TMRs) suggesting a "core" function in membrane traffic.
Five isoforms (SCAMP 1-5) have been described. SCAMP 1-3 contain NPF repeats that interact with EH-domain proteins which are involved in the budding of transport vesicles from the plasma membrane or the Golgi complex. SCAMP 4 and SCAMP 5 lack the NPF repeats.
SCAMP 1-4 are ubiquitously expressed whereas SCAMP 5 is expressed exclusively in brain during late development.

1 ) Fernández-Chacón R et al., J. Neurosci. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Fernández-Chacón R et al., J. Biol. Chem. (2000)
4 ) Fernández-Chacón R et al., Annu. Rev. Physiol. (1999)
5 ) Singleton DR et al., J. Cell. Sci. (1997)
6 ) Südhof TC et al., Nature (1995)
7 ) Brand SH et al., EMBO J. (1993)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

SCAMP1 antibody - 121 003

References for SCAMP1 - 121 003