SCAMP1 peptide - 121-0P

SCAMP 1 is an ubiquitous integral membrane protein of secretory and transport vesicles

control peptide

Cat. No.: 121-0P

Amount: 100 µg

Price: $105.00

Cat. No. 121-0P	100 µg peptide, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized!
Applications
Immunogen	Synthetic peptide corresponding to AA 2 to 15 from rat SCAMP1 (UniProt Id: P56603)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	121 002, 121 003
Remarks	This control peptide consists of the synthetic peptide (SDFDSNPFADPDLN) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	121-0p.pdf

Cat. No.: 121-0P

Amount: 100 µg

Price: $105.00

Background

SCAMPs (secretory carrier membrane proteins) are general markers of membranes that function in cell surface recycling such as secretory vesicles, pancreatic granules, etc. They have four conserved transmembrane regions (TMRs) suggesting a "core" function in membrane traffic.
Five isoforms (SCAMP 1-5) have been described. SCAMP 1-3 contain NPF repeats that interact with EH-domain proteins which are involved in the budding of transport vesicles from the plasma membrane or the Golgi complex. SCAMP 4 and SCAMP 5 lack the NPF repeats.
SCAMP 1-4 are ubiquitously expressed whereas SCAMP 5 is expressed exclusively in brain during late development.

1 ) Fernández-Chacón R et al., J. Neurosci. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Fernández-Chacón R et al., J. Biol. Chem. (2000)
4 ) Fernández-Chacón R et al., Annu. Rev. Physiol. (1999)
5 ) Singleton DR et al., J. Cell. Sci. (1997)
6 ) Südhof TC et al., Nature (1995)
7 ) Brand SH et al., EMBO J. (1993)

Protocols

OTHER

Preadsorbtion

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