Synaptic Systems

Cat. No.: 121 002

Amount: 200 µl

Price: $355.00

Cat. No. 121 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 1000 up to 1 : 5000 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 2 to 15 from rat SCAMP1 (UniProt Id: P56603)
Reactivity	Reacts with: human (O15126), rat (P56603), mouse (Q8K021), hamster. Other species not tested yet.
Matching control protein/peptide	121-0P
Data sheet	121_002.pdf

References for SCAMP1 - 121 002

SCAMP5 plays a critical role in synaptic vesicle endocytosis during high neuronal activity.
Zhao H, Kim Y, Park J, Park D, Lee SE, Chang I, Chang S
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3430: 10085-95. 121 002 WB; tested species: rat

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 121 002 ICC, IHC, WB; tested species: mouse,rat

The proteome of the presynaptic active zone: from docked synaptic vesicles to adhesion molecules and maxi-channels.
Morciano M, Beckhaus T, Karas M, Zimmermann H, Volknandt W
Journal of neurochemistry (2009) 1083: 662-75. 121 002 WB

Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 121 002 WB

Loss of the zymogen granule protein syncollin affects pancreatic protein synthesis and transport but not secretion.
Antonin W, Wagner M, Riedel D, Brose N, Jahn R
Molecular and cellular biology (2002) 225: 1545-54. 121 002 WB

SNARE proteins are highly enriched in lipid rafts in PC12 cells: implications for the spatial control of exocytosis.
Chamberlain LH, Burgoyne RD, Gould GW
Proceedings of the National Academy of Sciences of the United States of America (2001) 9810: 5619-24. 121 002 WB

The R-SNARE endobrevin/VAMP-8 mediates homotypic fusion of early endosomes and late endosomes.
Antonin W, Holroyd C, Tikkanen R, Höning S, Jahn R
Molecular biology of the cell (2000) 1110: 3289-98. 121 002 WB

Evidence for glutamate as a neuroglial transmitter within sensory ganglia.
Kung LH, Gong K, Adedoyin M, Ng J, Bhargava A, Ohara PT, Jasmin L
PloS one (2013) 87: e68312. 121 002 IHC

Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 121 002 IHC

Cat. No.: 121 002

Amount: 200 µl

Price: $355.00

Evidence for glutamate as a neuroglial transmitter within sensory ganglia.
Kung LH, Gong K, Adedoyin M, Ng J, Bhargava A, Ohara PT, Jasmin L
PloS one (2013) 87: e68312. 121 002 IHC

ICC

IHC

Background

SCAMPs (secretory carrier membrane proteins) are general markers of membranes that function in cell surface recycling such as secretory vesicles, pancreatic granules, etc. They have four conserved transmembrane regions (TMRs) suggesting a "core" function in membrane traffic.
Five isoforms (SCAMP 1-5) have been described. SCAMP 1-3 contain NPF repeats that interact with EH-domain proteins which are involved in the budding of transport vesicles from the plasma membrane or the Golgi complex. SCAMP 4 and SCAMP 5 lack the NPF repeats.
SCAMP 1-4 are ubiquitously expressed whereas SCAMP 5 is expressed exclusively in brain during late development.

1 ) Fernández-Chacón R et al., J. Neurosci. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Fernández-Chacón R et al., J. Biol. Chem. (2000)
4 ) Fernández-Chacón R et al., Annu. Rev. Physiol. (1999)
5 ) Singleton DR et al., J. Cell. Sci. (1997)
6 ) Südhof TC et al., Nature (1995)
7 ) Brand SH et al., EMBO J. (1993)

Protocols

Standard protocol

ICC

IHC

OTHER

Preabsorbtion

Other products for this target

SCAMP1 antibody - 121 002

References for SCAMP1 - 121 002