Cat. No.: 270-0P
Amount: 100 µg
Price:
$105.00
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| Cat. No. 270-0P |
100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
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| Immunogen | Recombinant protein corresponding to AA 70 to 154 from mouse Synaptotagmin11 (UniProt Id: Q9R0N3) |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Matching antibodies | 270 003 |
| Remarks |
This control protein consists of the recombinant protein (aa 70-154 of mouse synaptotagmin 11) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Data sheet | 270-0p.pdf |
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Up to now at least 17 synaptotagmins have been identified. Synaptotagmin11, a close relative of synaptotagmin4, is mainly expressed in the brain. It is composed of a vesicular, a transmembrane and two C2 domains. Only the C2B domain is able to bind calcium. In the C2A domain one of the calcium binding aspartates has been substituted for serine leading to a loss of its binding capabilities.
Synaptotagmin11 has been shown to localize mainly to excitatory and inhibitory presynaptic terminals.