Synaptic Systems - Synaptotagmin1

Cat. No.: 105 311C3

Amount: 100 µg

Price: $465.00

Cat. No. 105 311C3	100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 3. Fluorescence labeled antibodies conjugated to Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy. Cyanine 2: λ_ex 492 nm / λ_em 508 nm Sulfo-Cyanine 3: λ_ex 554 nm / λ_em 568 nm Sulfo-Cyanine 5: λ_ex 649 nm / λ_em 667 nm Cyanine 5 dyes are well suited for STORM high resolution microscopy. Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates. Albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: 1 : 50 up to 1 : 300 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet
Label	Sulfo-Cyanine 3
Clone	604.2
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity	Reacts with: rat (P21707). No signal: mouse (P46096), zebrafish. Other species not tested yet.
Remarks	ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.
Data sheet	105_311c3.pdf

References for Synaptotagmin1 - 105 311C3

Synaptic disruption and CREB-regulated transcription are restored by K+ channel blockers in ALS.
Catanese A, Rajkumar S, Sommer D, Freisem D, Wirth A, Aly A, Massa-López D, Olivieri A, Torelli F, Ioannidis V, Lipecka J, et al.
EMBO molecular medicine (2021) 137: e13131. 105 311C3 ICC; tested species: rat

Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing.
Lazarevic V, Schöne C, Heine M, Gundelfinger ED, Fejtova A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10189-200. 105 311C3 ICC

α-Synuclein induced cholesterol lowering increases tonic and reduces depolarization-evoked synaptic vesicle recycling and glutamate release.
Lazarevic V, Yang Y, Paslawski W, Svenningsson P
NPJ Parkinson's disease (2022) 81: 71. 105 311C3 UPTAKE; tested species: mouse

Aβ1-16 controls synaptic vesicle pools at excitatory synapses via cholinergic modulation of synapsin phosphorylation.
Anni D, Weiss EM, Guhathakurta D, Akdas YE, Klueva J, Zeitler S, Andres-Alonso M, Huth T, Fejtova A
Cellular and molecular life sciences : CMLS (2021) : . 105 311C3 UPTAKE; tested species: rat

The GTPase Rab26 links synaptic vesicles to the autophagy pathway.
Binotti B, Pavlos NJ, Riedel D, Wenzel D, Vorbrüggen G, Schalk AM, Kühnel K, Boyken J, Erck C, Martens H, Chua JJ, et al.
eLife (2015) 4: e05597. 105 311C3 UPTAKE

Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling.
Pan PY, Marrs J, Ryan TA
The Journal of biological chemistry (2015) 29037: 22593-601. 105 311C3 UPTAKE

GABA(A) receptors can initiate the formation of functional inhibitory GABAergic synapses.
Fuchs C, Abitbol K, Burden JJ, Mercer A, Brown L, Iball J, Anne Stephenson F, Thomson AM, Jovanovic JN
The European journal of neuroscience (2013) 388: 3146-58. 105 311C3 UPTAKE; tested species: rat

LRRK2 controls synaptic vesicle storage and mobilization within the recycling pool.
Piccoli G, Condliffe SB, Bauer M, Giesert F, Boldt K, De Astis S, Meixner A, Sarioglu H, Vogt-Weisenhorn DM, Wurst W, Gloeckner CJ, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 316: 2225-37. 105 311C3 UPTAKE

Dvl regulates endo- and exocytotic processes through binding to synaptotagmin.
Kishida S, Hamao K, Inoue M, Hasegawa M, Matsuura Y, Mikoshiba K, Fukuda M, Kikuchi A
Genes to cells : devoted to molecular & cellular mechanisms (2007) 121: 49-61. 105 311C3 UPTAKE; tested species: rat

Evidence for early endosome-like fusion of recently endocytosed synaptic vesicles.
Rizzoli SO, Bethani I, Zwilling D, Wenzel D, Siddiqui TJ, Brandhorst D, Jahn R
Traffic (Copenhagen, Denmark) (2006) 79: 1163-76. 105 311C3 UPTAKE; tested species: rat

Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 105 311C3

Cat. No.: 105 311C3

Amount: 100 µg

Price: $465.00

ICC

Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca²⁺-binding C2-domains.
Ca²⁺-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca²⁺-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.

1 ) Geppert M, Annual review of neuroscience (1998) 21: 75-95.
2 ) Jahn R, Annual review of neuroscience (1994) 17: 219-46.
3 ) Südhof TC, Nature (1995) 3756533: 645-53.
4 ) Geppert M, Cell (1994) 794: 717-27.
5 ) Brose N, Science (New York, N.Y.) (1992) 2565059: 1021-5.

Protocols

ICC

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Synaptotagmin1 antibody luminal domain - 105 311C3

References for Synaptotagmin1 - 105 311C3