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Home > Products > 105 104

Synaptotagmin1 antibody luminal domain - 105 104

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Guinea pig polyclonal antiserum
Cat. No.: 105 104
Amount: 100 µl
Price: $365.00
Cat. No. 105 104 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 2000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 5000 (see remarks) gallery  
IHC: 1 : 500 gallery  
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096)
Reactivity Reacts with: rat (P21707).
Other species not tested yet.
For unknown reasons antibodies raised against the luminal N-terminus of Synaptotagmin 1 show a strong preference for the rat protein.
Matching control protein/peptide 105-10P
Remarks

ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.
Data sheet 105_104.pdf

References for Synaptotagmin1 - 105 104

Neural Cell Adhesion Molecule 2 (NCAM2)-Induced c-Src-Dependent Propagation of Submembrane Ca2+ Spikes Along Dendrites Inhibits Synapse Maturation.
Sheng L, Leshchyns'ka I, Sytnyk V
Cerebral cortex (New York, N.Y. : 1991) (2018) : . 105 104 ICC; tested species: mouse
Neuronal profilin isoforms are addressed by different signalling pathways.
Murk K, Wittenmayer N, Michaelsen-Preusse K, Dresbach T, Schoenenberger CA, Korte M, Jockusch BM, Rothkegel M
PloS one (2012) 73: e34167. 105 104 ICC; tested species: rat
Cat. No.: 105 104
Amount: 100 µl
Price: $365.00
Neural Cell Adhesion Molecule 2 (NCAM2)-Induced c-Src-Dependent Propagation of Submembrane Ca2+ Spikes Along Dendrites Inhibits Synapse Maturation.
Sheng L, Leshchyns'ka I, Sytnyk V
Cerebral cortex (New York, N.Y. : 1991) (2018) : . 105 104 ICC; tested species: mouse
Neuronal profilin isoforms are addressed by different signalling pathways.
Murk K, Wittenmayer N, Michaelsen-Preusse K, Dresbach T, Schoenenberger CA, Korte M, Jockusch BM, Rothkegel M
PloS one (2012) 73: e34167. 105 104 ICC; tested species: rat
WB
ICC
IHC
Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.
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