Cat. No.: 105 103C3
Amount: 50 µg
Price:
$485.00
|
|
|
| Cat. No. 105 103C3 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with
Cyanine 3.
Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications |
IP: N/A ICC: 1 : 100 up to 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet |
| Label | Sulfo-Cyanine 3 |
| Immunogen | Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096) |
| Reactivity |
Reacts with: rat (P21707), mouse (P46096). Other species not tested yet. For unknown reasons antibodies raised against the luminal N-terminus of Synaptotagmin 1 show a strong preference for the rat protein. |
| Matching control protein/peptide | 105-10P |
| Remarks |
ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures. |
| Data sheet | 105_103c3.pdf |
|
|
Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.