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Synaptotagmin1 antibody luminal domain - 105 103 K.O.

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Rabbit polyclonal purified antibody
Cat. No.: 105 103
Amount: 50 µg
Price: $460.00
Cat. No. 105 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC: 1 : 500 (rat only) gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096)
Reactivity Reacts with: rat (P21707), mouse (P46096).
Other species not tested yet.

For unknown reasons antibodies raised against the luminal N-terminus of Synaptotagmin 1 show a strong preference for the rat protein.
Specificity K.O. validated PubMed: 31501440
Matching control protein/peptide 105-10P
Remarks

ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.

Data sheet 105_103.pdf

References for Synaptotagmin1 - 105 103

Monitoring of activity-driven trafficking of endogenous synaptic proteins through proximity labeling.
Pascual-Caro C, de Juan-Sanz J
PLoS biology (2024) 2210: e3002860. 105 103 WB; tested species: rat
Synaptotagmin 2 is ectopically overexpressed in excitatory presynapses of a widely used CaMKΙΙα-Cre mouse line.
Matsuura K, Mohamed HMA, Youssef MMM, Yoshida Y, Yamamoto T
iScience (2022) 258: 104692. 105 103 WB; tested species: mouse
Paroxysmal and cognitive phenotypes in Prrt2 mutant mice.
Robertson L, Featherby T, Howell S, Hughes J, Thomas P
Genes, brain, and behavior (2019) : e12566. 105 103 WB; tested species: mouse
Synaptophysin sustains presynaptic performance by preserving vesicular synaptobrevin-II levels.
Kokotos AC, Harper CB, Marland JRK, Smillie KJ, Cousin MA, Gordon SL
Journal of neurochemistry (2019) : . 105 103 WB, ICC; tested species: mouse
Synaptotagmin 1 clamps synaptic vesicle fusion in mammalian neurons independent of complexin.
Courtney NA, Bao H, Briguglio JS, Chapman ER
Nature communications (2019) 101: 4076. 105 103 WB; KO verified; tested species: mouse
Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts.
Raja MK, Preobraschenski J, Del Olmo-Cabrera S, Martinez-Turrillas R, Jahn R, Perez-Otano I, Wesseling JF
eLife (2019) 8: . 105 103 WB; tested species: mouse
Expression and secretion of synaptic proteins during stem cell differentiation to cortical neurons.
Nazir FH, Becker B, Brinkmalm A, Höglund K, Sandelius Å, Bergström P, Satir TM, Öhrfelt A, Blennow K, Agholme L, Zetterberg H, et al.
Neurochemistry international (2018) : . 105 103 WB, ICC; tested species: human
Simultaneous monitoring of presynaptic transmitter release and postsynaptic receptor trafficking reveals an enhancement of presynaptic activity in metabotropic glutamate receptor-mediated long-term depression.
Xu W, Tse YC, Dobie FA, Baudry M, Craig AM, Wong TP, Wang YT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3313: 5867-5877. 105 103 WB
Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice.
Delignat-Lavaud B, Kano J, Ducrot C, Massé I, Mukherjee S, Giguère N, Moquin L, Lévesque C, Burke S, Denis R, Bourque MJ, et al.
Nature communications (2023) 141: 4120. 105 103 ICC; KO verified; tested species: mouse
Phosphatidylinositol 4-kinase IIα is a glycogen synthase kinase 3-regulated interaction hub for activity-dependent bulk endocytosis.
Blumrich EM, Nicholson-Fish JC, Pronot M, Davenport EC, Kurian D, Cole A, Smillie KJ, Cousin MA
Cell reports (2023) 426: 112633. 105 103 ICC; tested species: rat
Endosomal phosphatidylinositol 3-phosphate controls synaptic vesicle cycling and neurotransmission.
Liu GT, Kochlamazashvili G, Puchkov D, Müller R, Schultz C, Mackintosh AI, Vollweiter D, Haucke V, Soykan T
The EMBO journal (2022) : e109352. 105 103 ICC; tested species: rat
Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons.
Vevea JD, Chapman ER
eLife (2020) 9: . 105 103 ICC; KO verified; tested species: mouse
Synaptophysin sustains presynaptic performance by preserving vesicular synaptobrevin-II levels.
Kokotos AC, Harper CB, Marland JRK, Smillie KJ, Cousin MA, Gordon SL
Journal of neurochemistry (2019) : . 105 103 WB, ICC; tested species: mouse
Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters.
Trotter JH, Hao J, Maxeiner S, Tsetsenis T, Liu Z, Zhuang X, Südhof TC
The Journal of cell biology (2019) : . 105 103 ICC; tested species: mouse
C-terminal calcium binding of α-synuclein modulates synaptic vesicle interaction.
Lautenschläger J, Stephens AD, Fusco G, Ströhl F, Curry N, Zacharopoulou M, Michel CH, Laine R, Nespovitaya N, Fantham M, Pinotsi D, et al.
Nature communications (2018) 91: 712. 105 103 ICC; tested species: rat
Expression and secretion of synaptic proteins during stem cell differentiation to cortical neurons.
Nazir FH, Becker B, Brinkmalm A, Höglund K, Sandelius Å, Bergström P, Satir TM, Öhrfelt A, Blennow K, Agholme L, Zetterberg H, et al.
Neurochemistry international (2018) : . 105 103 WB, ICC; tested species: human
Revisiting adult neurogenesis and the role of erythropoietin for neuronal and oligodendroglial differentiation in the hippocampus.
Hassouna I, Ott C, Wüstefeld L, Offen N, Neher RA, Mitkovski M, Winkler D, Sperling S, Fries L, Goebbels S, Vreja IC, et al.
Molecular psychiatry (2016) 2112: 1752-1767. 105 103 ICC
Control of exocytosis by synaptotagmins and otoferlin in auditory hair cells.
Beurg M, Michalski N, Safieddine S, Bouleau Y, Schneggenburger R, Chapman ER, Petit C, Dulon D
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13281-90. 105 103 IHC; tested species: rat
Cat. No.: 105 103
Amount: 50 µg
Price: $460.00
Monitoring of activity-driven trafficking of endogenous synaptic proteins through proximity labeling.
Pascual-Caro C, de Juan-Sanz J
PLoS biology (2024) 2210: e3002860. 105 103 WB; tested species: rat
Synaptotagmin 2 is ectopically overexpressed in excitatory presynapses of a widely used CaMKΙΙα-Cre mouse line.
Matsuura K, Mohamed HMA, Youssef MMM, Yoshida Y, Yamamoto T
iScience (2022) 258: 104692. 105 103 WB; tested species: mouse
Paroxysmal and cognitive phenotypes in Prrt2 mutant mice.
Robertson L, Featherby T, Howell S, Hughes J, Thomas P
Genes, brain, and behavior (2019) : e12566. 105 103 WB; tested species: mouse
Synaptophysin sustains presynaptic performance by preserving vesicular synaptobrevin-II levels.
Kokotos AC, Harper CB, Marland JRK, Smillie KJ, Cousin MA, Gordon SL
Journal of neurochemistry (2019) : . 105 103 WB, ICC; tested species: mouse
Synaptotagmin 1 clamps synaptic vesicle fusion in mammalian neurons independent of complexin.
Courtney NA, Bao H, Briguglio JS, Chapman ER
Nature communications (2019) 101: 4076. 105 103 WB; KO verified; tested species: mouse
Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts.
Raja MK, Preobraschenski J, Del Olmo-Cabrera S, Martinez-Turrillas R, Jahn R, Perez-Otano I, Wesseling JF
eLife (2019) 8: . 105 103 WB; tested species: mouse
Expression and secretion of synaptic proteins during stem cell differentiation to cortical neurons.
Nazir FH, Becker B, Brinkmalm A, Höglund K, Sandelius Å, Bergström P, Satir TM, Öhrfelt A, Blennow K, Agholme L, Zetterberg H, et al.
Neurochemistry international (2018) : . 105 103 WB, ICC; tested species: human
Simultaneous monitoring of presynaptic transmitter release and postsynaptic receptor trafficking reveals an enhancement of presynaptic activity in metabotropic glutamate receptor-mediated long-term depression.
Xu W, Tse YC, Dobie FA, Baudry M, Craig AM, Wong TP, Wang YT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3313: 5867-5877. 105 103 WB
Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice.
Delignat-Lavaud B, Kano J, Ducrot C, Massé I, Mukherjee S, Giguère N, Moquin L, Lévesque C, Burke S, Denis R, Bourque MJ, et al.
Nature communications (2023) 141: 4120. 105 103 ICC; KO verified; tested species: mouse
Phosphatidylinositol 4-kinase IIα is a glycogen synthase kinase 3-regulated interaction hub for activity-dependent bulk endocytosis.
Blumrich EM, Nicholson-Fish JC, Pronot M, Davenport EC, Kurian D, Cole A, Smillie KJ, Cousin MA
Cell reports (2023) 426: 112633. 105 103 ICC; tested species: rat
Endosomal phosphatidylinositol 3-phosphate controls synaptic vesicle cycling and neurotransmission.
Liu GT, Kochlamazashvili G, Puchkov D, Müller R, Schultz C, Mackintosh AI, Vollweiter D, Haucke V, Soykan T
The EMBO journal (2022) : e109352. 105 103 ICC; tested species: rat
Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons.
Vevea JD, Chapman ER
eLife (2020) 9: . 105 103 ICC; KO verified; tested species: mouse
Synaptophysin sustains presynaptic performance by preserving vesicular synaptobrevin-II levels.
Kokotos AC, Harper CB, Marland JRK, Smillie KJ, Cousin MA, Gordon SL
Journal of neurochemistry (2019) : . 105 103 WB, ICC; tested species: mouse
Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters.
Trotter JH, Hao J, Maxeiner S, Tsetsenis T, Liu Z, Zhuang X, Südhof TC
The Journal of cell biology (2019) : . 105 103 ICC; tested species: mouse
C-terminal calcium binding of α-synuclein modulates synaptic vesicle interaction.
Lautenschläger J, Stephens AD, Fusco G, Ströhl F, Curry N, Zacharopoulou M, Michel CH, Laine R, Nespovitaya N, Fantham M, Pinotsi D, et al.
Nature communications (2018) 91: 712. 105 103 ICC; tested species: rat
Expression and secretion of synaptic proteins during stem cell differentiation to cortical neurons.
Nazir FH, Becker B, Brinkmalm A, Höglund K, Sandelius Å, Bergström P, Satir TM, Öhrfelt A, Blennow K, Agholme L, Zetterberg H, et al.
Neurochemistry international (2018) : . 105 103 WB, ICC; tested species: human
Revisiting adult neurogenesis and the role of erythropoietin for neuronal and oligodendroglial differentiation in the hippocampus.
Hassouna I, Ott C, Wüstefeld L, Offen N, Neher RA, Mitkovski M, Winkler D, Sperling S, Fries L, Goebbels S, Vreja IC, et al.
Molecular psychiatry (2016) 2112: 1752-1767. 105 103 ICC
Control of exocytosis by synaptotagmins and otoferlin in auditory hair cells.
Beurg M, Michalski N, Safieddine S, Bouleau Y, Schneggenburger R, Chapman ER, Petit C, Dulon D
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13281-90. 105 103 IHC; tested species: rat
Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.