Synaptic Systems - Synaptotagmin1/2

Cat. No.: 105 003

Amount: 50 µg

Price: $455.00

Cat. No. 105 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: not tested yet ELISA: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen	Synthetic peptide corresponding to AA 120 to 131 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity	Reacts with: human (P21579), rat (P21707), mouse (P46096), cow, chicken, goldfish, zebrafish. Other species not tested yet.
Specificity	Some cross-reactivity to synaptotagmin 2. K.O. validated PubMed: 32015138
Matching control protein/peptide	105-0P
Remarks	ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 105 011 as capture antibodies. The ELISA-protocol for membrane proteins is recommended.
Data sheet	105_003.pdf

References for Synaptotagmin1/2 - 105 003

Synaptotagmin 1 oligomers clamp and regulate different modes of neurotransmitter release.
Tagliatti E, Bello OD, Mendonça PRF, Kotzadimitriou D, Nicholson E, Coleman J, Timofeeva Y, Rothman JE, Krishnakumar SS, Volynski KE
Proceedings of the National Academy of Sciences of the United States of America (2020) : . 105 003 WB, ICC; KO verified; tested species: mouse

Monocytic Infiltrates Contribute to Autistic-like Behaviors in a Two-Hit Model of Neurodevelopmental Defects.
Chen HR, Chen CW, Mandhani N, Short-Miller JC, Smucker MR, Sun YY, Kuan CY
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4049: 9386-9400. 105 003 WB; tested species: mouse

Hevin-calcyon interaction promotes synaptic reorganization after brain injury.
Kim JH, Jung HG, Kim A, Shim HS, Hyeon SJ, Lee YS, Han J, Jung JH, Lee J, Ryu H, Park JY, et al.
Cell death and differentiation (2021) 289: 2571-2588. 105 003 ICC; tested species: mouse

Riluzole attenuates the efficacy of glutamatergic transmission by interfering with the size of the readily releasable neurotransmitter pool.
Lazarevic V, Yang Y, Ivanova D, Fejtova A, Svenningsson P
Neuropharmacology (2018) : . 105 003 ICC; tested species: rat

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 105 003 ICC; tested species: rat

Cell Types Promoting Goosebumps Form a Niche to Regulate Hair Follicle Stem Cells.
Shwartz Y, Gonzalez-Celeiro M, Chen CL, Pasolli HA, Sheu SH, Fan SM, Shamsi F, Assaad S, Lin ET, Zhang B, Tsai PC, et al.
Cell (2020) : . 105 003 IHC; tested species: mouse

Cat. No.: 105 003

Amount: 50 µg

Price: $455.00

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 105 003 ICC; tested species: rat

ICC

IHC

Background

Synaptotagmin1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca²⁺-binding C2-domains.
Ca²⁺-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca²⁺-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.

1 ) Geppert M et al., Annu. Rev. Neurosci. (1998)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Südhof TC et al., Nature (1995)
4 ) Geppert M et al., Cell (1994)
5 ) Brose N et al., Science (1992)
6 ) Perin MS et al., Nature (1990)

Protocols

Standard protocol

ICC

IHC

ELISA

Membrane proteins

OTHER

Preabsorbtion

Other products for this target

Synaptotagmin1/2 antibody cytoplasmic tail - 105 003 K.O.

References for Synaptotagmin1/2 - 105 003