Cat. No.: 140-03P
Amount: 100 µg
Price:
$105.00
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| Cat. No. 140-03P |
100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
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| Immunogen | Recombinant protein corresponding to AA 909 to 1076 from rat RIM2-4C (UniProt Id: Q9JIS1-3) |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Matching antibodies | 140 303 |
| Remarks |
This control protein consists of the recombinant protein (aa 909 - 1076 of rat RIM 2) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Data sheet | 140-03p.pdf |
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RIMs are presynaptic active zone proteins that regulate Ca2+ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 2β consists of a specific N-terminus, the central PDZ domain and the C-terminal C2 domains. The mRNA for RIM 2β is transcribed from an internal promoter of the RIM 2α gene.
Shorter variants of RIM 2 which comprise only the C-terminal C2B domain and some flanking regions are referred to as NIM 2 / RIM 2γ and NIM 3 / RIM 3γ.