Cat. No.: 118-0P
Amount: 100 µg
Price:
$105.00
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| Cat. No. 118-0P |
100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized! |
| Applications |
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| Immunogen | Synthetic peptide corresponding to AA 671 to 684 from rat Rabphilin3a (UniProt Id: P47709) |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Matching antibodies | 118 002, 118 003 |
| Remarks |
This control peptide consists of the synthetic peptide (WHQLQNENHVSSD) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Data sheet | 118-0p.pdf |
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Rabphilin 3a is a putative effector protein for the low molecular weight GTP-binding protein rab 3. Rab 3 occurs in four isoforms (Rab 3a, b, c and d), all of which probably bind to rabphilin 3a when in the GTP-bound form.
Rabphilin 3a contains an N-terminal Zn2+-finger sequence that is essential for binding rab 3, and two C-terminal C2 - domains that may bind Ca2+. It does not have a transmembrane region.
Rabphilin 3a is primarily expressed in neurons where it is localized to synaptic vesicles. It is probably recruited to synaptic vesicles by rab 3a and 3c. The structure of rabphilin 3a and its interaction with rab 3 suggests that it may be a Ca2+ sensor on synaptic vesicles that is recruited to synaptic vesicles as a function of GTP by rab 3.