Synaptic Systems - Rabphilin3a

Cat. No.: 118 003

Amount: 50 µg

Price: $460.00

Cat. No. 118 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in Tris buffer. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) gallery IP: not recommended ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 671 to 684 from rat Rabphilin3a (UniProt Id: P47709)
Reactivity	Reacts with: human (Q9Y2J0), rat (P47709), mouse (P47708), cow. Other species not tested yet.
Matching control protein/peptide	118-0P
Data sheet	118_003.pdf

References for Rabphilin3a - 118 003

The proteome of the presynaptic active zone: from docked synaptic vesicles to adhesion molecules and maxi-channels.
Morciano M, Beckhaus T, Karas M, Zimmermann H, Volknandt W
Journal of neurochemistry (2009) 1083: 662-75. 118 003 WB; tested species: rat

Biochemical, molecular and behavioral phenotypes of Rab3A mutations in the mouse.
Yang S, Farias M, Kapfhamer D, Tobias J, Grant G, Abel T, Bućan M
Genes, brain, and behavior (2007) 61: 77-96. 118 003 WB; tested species: mouse

Cat. No.: 118 003

Amount: 50 µg

Price: $460.00

Background

Rabphilin 3a is a putative effector protein for the low molecular weight GTP-binding protein rab 3. Rab 3 occurs in four isoforms (Rab 3a, b, c and d), all of which probably bind to rabphilin 3a when in the GTP-bound form.
Rabphilin 3a contains an N-terminal Zn²⁺-finger sequence that is essential for binding rab 3, and two C-terminal C2 - domains that may bind Ca²⁺. It does not have a transmembrane region.
Rabphilin 3a is primarily expressed in neurons where it is localized to synaptic vesicles. It is probably recruited to synaptic vesicles by rab 3a and 3c. The structure of rabphilin 3a and its interaction with rab 3 suggests that it may be a Ca²⁺ sensor on synaptic vesicles that is recruited to synaptic vesicles as a function of GTP by rab 3.

1 ) Schlüter OM et al., J. Neurosci. (1999)
2 ) Fernández-Chacón R et al., Annu. Rev. Physiol. (1999)
3 ) Stahl B et al., EMBO J. (1996)
4 ) Li C et al., Neuron (1994)
5 ) Shirataki H et al., Mol. Cell. Biol. (1993)

Protocols

OTHER

Preabsorbtion

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Rabphilin3a antibody - 118 003

References for Rabphilin3a - 118 003