Rab5 antibody - 108 002

Rab5 is a GTP-binding protein involved in endocytosis

Rabbit polyclonal antiserum

Cat. No.: 108 002

Amount: 200 µl

Price: $355.00

Cat. No. 108 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 192 to 196 from mouse Rab5 (UniProt Id: Q9CQD1)
Reactivity	Reacts with: human (Q9UJ41), rat (M0RC99), mouse (Q9JM13). Other species not tested yet.
Matching control protein/peptide	108-0P
Data sheet	108_002.pdf

References for Rab5 - 108 002

Effects of phosphorylation and neuronal activity on the control of synapse formation by synapsin I.
Perlini LE, Botti F, Fornasiero EF, Giannandrea M, Bonanomi D, Amendola M, Naldini L, Benfenati F, Valtorta F
Journal of cell science (2011) 124Pt 21: 3643-53. 108 002 WB

Cat. No.: 108 002

Amount: 200 µl

Price: $355.00

Background

Rab 5 is a member of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. Rab 5 is ubiquitously expressed in all tissues where it functions in the fusion of early endosomes which is the first fusion step of endocytic organelles after their formation and detachment from the plasma membrane. It is presently the best marker with selectivity for this compartment.

1 ) Somsel Rodman J et al., J. Cell. Sci. (2000)
2 ) Ikin AF et al., J. Biol. Chem. (1996)

Protocols

Standard protocol

OTHER

Preabsorbtion

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