Synaptic Systems

Cat. No.: 107-1P

Amount: 100 µg

Price: $105.00

Background

Rab 3 is a member of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. Four related isoforms of Rab 3 are known (Rab 3a, 3b, 3c, and 3d). Rab 3a and 3c are predominantly expressed in neurons and neuroendocrine cells where they are localized to synaptic vesicles. Unlike the integral membrane proteins of synaptic vesicles, Rab 3a/c is absent from the Golgi complex and thus does not result in immunostaining of the axo-dendritic region as sometimes seen with e.g. synaptophysin, synaptobrevin/VAMP, or synaptogyrin.
Rab 3b and 3d are expressed in non-neuronal tissues such as adipocytes and the exocrine pancreas (3d). It has been shown that overexpression of Rab 3 inhibits Ca²⁺ regulated excocytosis and converts it into an constitutive Ca²⁺ independent exocytosis mechanism.

1 ) Geppert M et al., Annu. Rev. Neurosci. (1998)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Geppert M et al., Nature (1997)
4 ) Südhof TC et al., Nature (1995)
5 ) Fischer von Mollard G et al., Eur. J. Cell Biol. (1994)
6 ) Chen X et al., J. Biol. Chem. (2003)
7 ) Millar AL et al., Histol. Histopathol. (2002)
8 ) Pavlos NJ et al., J. Mol. Endocrinol. (2001)
9 ) Lin CG et al., Biochem. J. (1997)
10 ) Fischer von Mollard G et al., J. Biol. Chem. (1994)

Protocols

OTHER

Preadsorbtion

Other products for this target

Cat. No. 107-1P	100 µg peptide, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized!
Applications
Immunogen	Synthetic peptide corresponding to AA 2 to 14 from rat Rab3a (UniProt Id: P63012)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	107 102
Remarks	This control peptide consists of the synthetic peptide (ASATDSRYGQKES) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	107-1p.pdf

Rab3a peptide - 107-1P