Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 168 013

Rab27A antibody - 168 013 K.O.

Rab27 proteins are members of the Rab family responsible for melanosomal transport and hormone secretion
Rabbit polyclonal purified antibody
Cat. No.: 168 013
Amount: 50 µg
Price: $375.00
Cat. No. 168 013 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 1 to 221 from rat Rab27A (UniProt Id: P23640)
Reactivity Reacts with: rat (P23640), mouse (Q9ERI2), human (P51159).
Other species not tested yet.
Specificity Specific for rab 27A. No cross reactivity to rab 27B. K.O. validated PubMed: 25951179
Matching control protein/peptide 168-0P
Remarks

Only the non-conserved C-terminal part of the protein was used for affinity purification to achieve specificity.
Data sheet 168_013.pdf

References for Rab27A - 168 013

Kinesin-1: A New Actor Involved in Platelet Secretion and Thrombus Stability.
Adam F, Kauskot A, Kurowska M, Goudin N, Munoz I, Bordet JC, Huang JD, Bryckaert M, Fischer A, Borgel D, de Saint Basile G, et al.
Arteriosclerosis, thrombosis, and vascular biology (2018) : . 168 013 WB; tested species: mouse
Genome-wide interrogation of extracellular vesicle biology using barcoded miRNAs.
Lu A, Wawro P, Morgens DW, Portela F, Bassik MC, Pfeffer SR
eLife (2018) 7: . 168 013 WB; KD verified; tested species: human
UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells.
Maschalidi S, Nunes-Hasler P, Nascimento CR, Sallent I, Lannoy V, Garfa-Traore M, Cagnard N, Sepulveda FE, Vargas P, Lennon-Duménil AM, van Endert P, et al.
Nature communications (2017) 81: 1640. 168 013 WB, Dot blot; tested species: mouse
MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation.
Yoon S, Kovalenko A, Bogdanov K, Wallach D
Immunity (2017) 471: 51-65.e7. 168 013 WB; KD verified; tested species: human
Genetic deletion of Rab27B in pancreatic acinar cells affects granules size and has inhibitory effects on amylase secretion.
Hou Y, Ernst SA, Lentz SI, Williams JA
Biochemical and biophysical research communications (2016) 4714: 610-5. 168 013 WB
Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion.
Hou Y, Ernst SA, Stuenkel EL, Lentz SI, Williams JA
PloS one (2015) 105: e0125596. 168 013 WB, IHC; KO verified
Alterations in microRNA expression contribute to fatty acid-induced pancreatic beta-cell dysfunction.
Lovis P, Roggli E, Laybutt DR, Gattesco S, Yang JY, Widmann C, Abderrahmani A, Regazzi R
Diabetes (2008) 5710: 2728-36. 168 013 WB
UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells.
Maschalidi S, Nunes-Hasler P, Nascimento CR, Sallent I, Lannoy V, Garfa-Traore M, Cagnard N, Sepulveda FE, Vargas P, Lennon-Duménil AM, van Endert P, et al.
Nature communications (2017) 81: 1640. 168 013 WB, Dot blot; tested species: mouse
Rab27a Contributes to the Processing of Inflammatory Pain in Mice.
Gross T, Wack G, Syhr KMJ, Tolmachova T, Seabra MC, Geisslinger G, Niederberger E, Schmidtko A, Kallenborn-Gerhardt W
Cells (2020) 96: . 168 013 IHC; tested species: mouse
Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion.
Hou Y, Ernst SA, Stuenkel EL, Lentz SI, Williams JA
PloS one (2015) 105: e0125596. 168 013 WB, IHC; KO verified
Cat. No.: 168 013
Amount: 50 µg
Price: $375.00
Kinesin-1: A New Actor Involved in Platelet Secretion and Thrombus Stability.
Adam F, Kauskot A, Kurowska M, Goudin N, Munoz I, Bordet JC, Huang JD, Bryckaert M, Fischer A, Borgel D, de Saint Basile G, et al.
Arteriosclerosis, thrombosis, and vascular biology (2018) : . 168 013 WB; tested species: mouse
Genome-wide interrogation of extracellular vesicle biology using barcoded miRNAs.
Lu A, Wawro P, Morgens DW, Portela F, Bassik MC, Pfeffer SR
eLife (2018) 7: . 168 013 WB; KD verified; tested species: human
UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells.
Maschalidi S, Nunes-Hasler P, Nascimento CR, Sallent I, Lannoy V, Garfa-Traore M, Cagnard N, Sepulveda FE, Vargas P, Lennon-Duménil AM, van Endert P, et al.
Nature communications (2017) 81: 1640. 168 013 WB, Dot blot; tested species: mouse
MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation.
Yoon S, Kovalenko A, Bogdanov K, Wallach D
Immunity (2017) 471: 51-65.e7. 168 013 WB; KD verified; tested species: human
Genetic deletion of Rab27B in pancreatic acinar cells affects granules size and has inhibitory effects on amylase secretion.
Hou Y, Ernst SA, Lentz SI, Williams JA
Biochemical and biophysical research communications (2016) 4714: 610-5. 168 013 WB
Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion.
Hou Y, Ernst SA, Stuenkel EL, Lentz SI, Williams JA
PloS one (2015) 105: e0125596. 168 013 WB, IHC; KO verified
Alterations in microRNA expression contribute to fatty acid-induced pancreatic beta-cell dysfunction.
Lovis P, Roggli E, Laybutt DR, Gattesco S, Yang JY, Widmann C, Abderrahmani A, Regazzi R
Diabetes (2008) 5710: 2728-36. 168 013 WB
UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells.
Maschalidi S, Nunes-Hasler P, Nascimento CR, Sallent I, Lannoy V, Garfa-Traore M, Cagnard N, Sepulveda FE, Vargas P, Lennon-Duménil AM, van Endert P, et al.
Nature communications (2017) 81: 1640. 168 013 WB, Dot blot; tested species: mouse
Rab27a Contributes to the Processing of Inflammatory Pain in Mice.
Gross T, Wack G, Syhr KMJ, Tolmachova T, Seabra MC, Geisslinger G, Niederberger E, Schmidtko A, Kallenborn-Gerhardt W
Cells (2020) 96: . 168 013 IHC; tested species: mouse
Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion.
Hou Y, Ernst SA, Stuenkel EL, Lentz SI, Williams JA
PloS one (2015) 105: e0125596. 168 013 WB, IHC; KO verified
WB
Background
Rab 27 proteins are members of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. These proteins are involved in intracellular fusion reactions of vesicles or organelles with their target membranes. Two Rab 27 isoforms, Rab 27A and 27B, have been described so far.
Mutations in the Rab 27A gene have been shown to be responsible for the Griscelli syndrome characterized by pigment dilution of the hair and an uncontrolled T-lymphocyte and macrophage activation. This disorder is probably due to the dysfunction of melanosomes in melanocytes and lytic granules in CTLs. Additionally Rab 27A is located on mature insulin granules of pancreatic β-cells and is expressed in the pigment epithelium and choriocapillaris of the retina.
In patients who suffer from Griscelli syndrome because of missense mutations in the Rab 27A gene, Rab 27B is upregulated and partially compensates for Rab 27A dysfunction. Rab 27B also regulates amylase secretion in parotid acinar cells.
Recently it has been shown that Rab 27 is also involved in synaptic transmission in C. elegans.
Other products for this target