Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 168 003

Rab27 antibody - 168 003

Rab27 proteins are members of the Rab family responsible for melanosomal transport and hormone secretion
Rabbit polyclonal purified antibody
Cat. No.: 168 003
Amount: 50 µg
Price: $375.00
Cat. No. 168 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 1 to 221 from rat Rab27A (UniProt Id: P23640)
Reactivity Reacts with: rat (P23640, Q99P74), mouse (Q9ERI2, Q99P58).
Other species not tested yet.
Specificity Recognizes both isoforms rab 27A and B; no cross reaction to other rab proteins.
Matching control protein/peptide 168-0P
Data sheet 168_003.pdf

References for Rab27 - 168 003

UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells.
Maschalidi S, Nunes-Hasler P, Nascimento CR, Sallent I, Lannoy V, Garfa-Traore M, Cagnard N, Sepulveda FE, Vargas P, Lennon-Duménil AM, van Endert P, et al.
Nature communications (2017) 81: 1640. 168 003 WB; tested species: mouse
Synaptotagmin-1 docks secretory vesicles to syntaxin-1/SNAP-25 acceptor complexes.
de Wit H, Walter AM, Milosevic I, Gulyás-Kovács A, Riedel D, Sørensen JB, Verhage M
Cell (2009) 1385: 935-46. 168 003 WB
Phosphorylation negatively regulates exosome mediated secretion of cryAB in glioma cells.
Kore RA, Abraham EC
Biochimica et biophysica acta (2016) 18632: 368-77. 168 003 ICC
Cat. No.: 168 003
Amount: 50 µg
Price: $375.00
UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells.
Maschalidi S, Nunes-Hasler P, Nascimento CR, Sallent I, Lannoy V, Garfa-Traore M, Cagnard N, Sepulveda FE, Vargas P, Lennon-Duménil AM, van Endert P, et al.
Nature communications (2017) 81: 1640. 168 003 WB; tested species: mouse
Synaptotagmin-1 docks secretory vesicles to syntaxin-1/SNAP-25 acceptor complexes.
de Wit H, Walter AM, Milosevic I, Gulyás-Kovács A, Riedel D, Sørensen JB, Verhage M
Cell (2009) 1385: 935-46. 168 003 WB
Phosphorylation negatively regulates exosome mediated secretion of cryAB in glioma cells.
Kore RA, Abraham EC
Biochimica et biophysica acta (2016) 18632: 368-77. 168 003 ICC
WB
Background
Rab 27 proteins are members of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. These proteins are involved in intracellular fusion reactions of vesicles or organelles with their target membranes. Two Rab 27 isoforms, Rab 27A and 27B, have been described so far.
Mutations in the Rab 27A gene have been shown to be responsible for the Griscelli syndrome characterized by pigment dilution of the hair and an uncontrolled T-lymphocyte and macrophage activation. This disorder is probably due to the dysfunction of melanosomes in melanocytes and lytic granules in CTLs. Additionally Rab 27A is located on mature insulin granules of pancreatic β-cells and is expressed in the pigment epithelium and choriocapillaris of the retina.
In patients who suffer from Griscelli syndrome because of missense mutations in the Rab 27A gene, Rab 27B is upregulated and partially compensates for Rab 27A dysfunction. Rab 27B also regulates amylase secretion in parotid acinar cells.
Recently it has been shown that Rab 27 is also involved in synaptic transmission in C. elegans.