Cat. No.: 116-2P
Amount: 100 µg
Price:
$105.00
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| Cat. No. 116-2P |
100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized! |
| Applications |
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| Immunogen | Synthetic peptide corresponding to AA 575 to 593 from rat Munc18-3 (UniProt Id: Q99PV2) |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Matching antibodies | 116 202, 116 203 |
| Remarks |
This control peptide consists of the synthetic peptide (aa 575 - 593 in rat Munc 18-3) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Data sheet | 116-2p.pdf |
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Munc 18 is an abundant neuronal protein that tightly binds to the synaptic fusion protein syntaxin 1. It is highly homologous to the C. elegans unc-18 gene product, and weakly related to the yeast sec1, sly1, and slp1 genes.
There are three munc 18 isoforms in mammals. Munc 18-1 or 18a, also referred to as rb-sec1, n-sec1, stxbp1 and p67, is primarily expressed in neurons. Munc 18-2 or 18b, also referred to as stxbp2, and Munc 18-3 or 18c are expressed ubiquitously.