Cat. No.: 188 003
Amount: 50 µg
Price:
$455.00
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| Cat. No. 188 003 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Recombinant protein corresponding to residues near the amino terminus of human Map2 (UniProt Id: P11137-4) |
| Reactivity |
Reacts with: human (P11137), rat (P15146), mouse (P20357). Other species not tested yet. |
| Specificity | Specific for MAP2; recognizes all four isoforms. |
| Matching control protein/peptide | 188-0P |
| Remarks |
WB: Due to its large size, MAP2 requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen. |
| Data sheet | 188_003.pdf |
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There are two major classes of heat stable microtubule associated proteins (MAPs): MAP2, and tau. Both protein classes are involved in the regulation of microtubule polymerization in cells.
Four differentially regulated isoforms of MAP2 have been described so far.