Synaptic Systems

Cat. No.: 229 003

Amount: 50 µg

Price: $375.00

Cat. No. 229 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 200 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 13 to 21 from rat MAL2A (UniProt Id: Q7TPB7)
Reactivity	Reacts with: rat (Q7TPB7). Other species not tested yet.
Matching control protein/peptide	229-0P
Data sheet	229_003.pdf

References for MAL2A - 229 003

Quantitative comparison of glutamatergic and GABAergic synaptic vesicles unveils selectivity for few proteins including MAL2, a novel synaptic vesicle protein.
Grønborg M, Pavlos NJ, Brunk I, Chua JJ, Münster-Wandowski A, Riedel D, Ahnert-Hilger G, Urlaub H, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 301: 2-12. 229 003 WB, ICC, IHC

Cat. No.: 229 003

Amount: 50 µg

Price: $375.00

ICC

IHC

Background

MAL 2 is an integral membrane protein that contains 4 putative transmembrane domains and belongs to the myelin and lymphocyte (MAL) protein family. Two isoforms, MAL 2A and MAL 2B, have been described so far. MAL 2 overexpression has been reported in several cancer types like breast, ovarian, and pancreatic carcinoma. It has been shown to be an essential component of the machinery for basolateral-to-apical transcytosis in hepatoma HepG2 cells but its mechanistic function still has to be elucidated.
Recently MAL 2A has been identified as a synaptic vesicle protein mainly localized to VGluT positive vesicles.

1 ) Fanayan S et al., BMC Cell Biol. (2009)
2 ) Marazuela M et al., J. Histochem. Cytochem. (2004)
3 ) Marazuela M et al., Endocrinology (2004)
4 ) de Marco MC et al., J. Cell Biol. (2002)

Protocols

ICC

IHC

OTHER

Preabsorbtion

Other products for this target

MAL2A antibody - 229 003

References for MAL2A - 229 003